Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1995-03-20
1997-07-01
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C12N 910
Patent
active
056437757
ABSTRACT:
This invention provides highly thermally stable trehalose phosphorylases. The trehalose phosphorylases of the present invention can be obtained from cultured mixture of microorganisms and are highly thermally stable, and produce .alpha.-D-glucose 1-phosphate and D-glucose by an action on trehalose and an inorganic phosphoric acid or salt thereof, or trehalose by an action on .alpha.-D-glucose 1-phosphate and D-glucose. This invention also relates to the process for the preparation of trehalose phosphorylase, and trehalose or .alpha.-D-glucose 1-phosphate with said trehalose phosphorylase.
REFERENCES:
Sharon L. Haynie et al., "Enzyme-Catalyzed Organic Synthesis of Sucrose and Trehalose with In Situ Regeneration of UDP-Glucose", Applied Biochemistry and Biotechnology, 23:155-170 (1990).
Kathleen A. Killick, "A Radiometric Assay for Trehalose-6-Phosphate Synthetase", Analytical Biochemistry, 79:310-318 (1977).
Yutaka Kitamoto et al., ".alpha.-Glucose-1-Phosphate Formation by a Novel Trehalose Phosphorylase from Flammulina Velutipes", FEMS Microbiology Letters, 55:147-150 (1988).
L.R. Marechal et al., "Metabolism of Trehalose in Euglena Gracilis" J. Biol. Chem. 247 No. 10:3223-3228 (1972).
S.O. Salminen et al., "Enzymes of .alpha.,.alpha.-Trehalose Metabolism in Soybean Nodules" Plant Physiol. 81:538-541 (1986).
Konai Yutaka
Saitoh Kohki
Takahashi Eiichi
Takahashi Eisaku
Wada Toshihiko
Kureha Chemical Industry Co. Ltd.
Lilling Herbert J.
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