Treatment of yeast cells with proteolytic enzymes

Details Treatment of yeast cells with proteolytic enzymes Treatment of yeast cells with proteolytic enzymes

1983-10-17

1985-12-17

Naff, David M.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

426 60, 435 69, 435222, 435255, 435256, 435267, 435938, C07K 312, A23L 128, C12N 116

Patent

active

045593078

ABSTRACT:
Functional protein having reduced nucleic acid content is produced without initial denaturation of the protein by contacting undenatured yeast cells with an alkaline protease at a temperature of about 20.degree. C. to 40.degree. C. for about 2 minutes to 2 hours at a pH of about 8 to 11. The yeast cells are preferably Pichia pastoris and the alkaline protease is preferably from Bacillus lichenformis.

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