Treatment of substrates for immobilizing biomolecules

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023100, C536S024300

Reexamination Certificate

active

10729619

ABSTRACT:
A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

REFERENCES:
patent: 5610287 (1997-03-01), Nikiforov et al.
patent: 5718898 (1998-02-01), Broeker et al.
patent: 5807522 (1998-09-01), Brown et al.
patent: 6110426 (2000-08-01), Shalon et al.
patent: 6171794 (2001-01-01), Burchard et al.
patent: 6800728 (2004-10-01), Schwartz
patent: WO2002033412 (2002-04-01), None
“Super Microarray Substrates Handbook”, www.arrayit.com, TeleChem International, Inc.
B. Clancy et al., “Reduction of Background Autofluorescence in Brain Sections Following Immersion in Sodium Borohydride”. Journal of Neuroscience Methods, vol. 83 (1998), pp. 97-102.
W. Beisker et al.., “An Improved Immunocytochemical Procedure for High-Sensitivity Detection of Incorporated Bromodeoxyuridine”, Cytometry, vol. 8 (1987), pp. 235-239.
S.A. Schnell et al., “Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue”, Journal of Histochemistry and Cytochemistry, vol. 47, Jun. 1999, pp. 719-730.
M.C. Willingham, “An Alternative Fixation-Processing Method For Preembedding Ultrastructural Immunocytochemistry of Cytoplasmic Antigens: The GBS (glutaraldehyde-borohydride-saponin) Procedure”, Journal of Histochemistry & Cytochemistry, vol. 31, Issue 6, Jun. 1, 1983, pp. 791-798.
M. Schena et al., “Parallel Human Genome Analysis: Microarray-Based Expression Monitoring of 1000 Genes”, Proc. Natl. Acad. Sci. USA, vol. 93, Oct. 1996, Biochemistry, pp. 10614-10619.
N. Zammateo et al., “Comparison Between Different Strategies of Covalent Attachment of DNA to Glass Surfaces to Build DNA Microarrays”, Analytical Biochemistry, vol. 280, 2000, pp. 143-150.
F. Diehl et al., “Manufacturing DNA Microarrays of High Spot Homogeneity and Reduced Background Signal”, Nucleic Acids Research, 2001, vol. 29, No. 7: E38, http:/
ar.oupjournals.org/cgi/content/full/29/7/e38.
Joon-Ik Ahn et al., Optimization of DNA Fabrication in Microarrays, Department of Biochemistry, College of Medicine & The Mental Health Institute, Hanyang University, Seoul.
Xenopore Binding Protocols, “Suggested Binding Protocols for Xenoslides”, http://www.xenopore.com/binding.htm.
Reduction Reactions, “II Reduction Reactions”, http://www.che.bham.ac.uk/publicsite/staff/staff%20details/cox/teaching/reduction%20react . . . .
“Sodium Borohydride Reduction”, http://ice.chem.umbc.edu/Chem3521/borohydride.html.
Schena et al., PNAS, USA, 93 : 10,614 - 10,619 (Oct. 1996).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Treatment of substrates for immobilizing biomolecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Treatment of substrates for immobilizing biomolecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Treatment of substrates for immobilizing biomolecules will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3958382

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.