Transposon mediated multiplex sequencing

Details Transposon mediated multiplex sequencing Transposon mediated multiplex sequencing

2007-06-12

2007-06-12

Lu, Frank (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091100, C536S023100, C536S024300, C536S024330, C536S025300, C536S025320

Reexamination Certificate

active

10332834

ABSTRACT:
The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

REFERENCES:
patent: WO 97/46714 (1997-12-01), None
patent: WO 98/37205 (1998-08-01), None
patent: WO 00/26243 (2000-05-01), None
BD AdvantageTM 2 PCR Enzyme System User Manual, pp. 1-24, Apr. 11, 2003.
S.E. Devine et al., Efficient integration of artificial transposons into plasmid targets in vitro: a useful tool for DNA mapping, sequeincing and genetic analysis, Nucleic Acids Research, vol. 22, No. 18, 1994, pp. 3765-3772.

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