Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1983-05-04
1986-12-23
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435317, 935 23, 935 38, 935 56, 935 73, C12P 2100, C12N 100, C12N 1500
Patent
active
046312595
ABSTRACT:
Processes and new genetic materials are provided for cloning specific DNA fragments by using a unique conjugative transposon designated Tn916. The transposon is used to first target specific genes by insertional inactivation. A restriction fragment containing the inserted transposon is then inserted into a plasmid vector and transformed into Escherichia coli or other suitable host by selection for the transposon encoded tetracycline (Tc) resistance. The transformants so produced are then grown in the absence of tetracycline conditions under which Tn916 excises from the chimeric plasmid thus restoring the integrity of the DNA into which the transposon was originally inserted. This process provides a new and useful way of producing new life forms that are useful for making desired products having established utility.
REFERENCES:
patent: 4237224 (1980-05-01), Cohen-Buyer
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Ike et al., J Bact, vol. 158, pp. 777-783, 1984, "Genetic Analysis of the PaD1 Pheromune Response in Streptococcus Faecalis, using Transposon Tn917 as an Insertional Mutagen".
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Clewell Don B.
Gawron-Burke Mary C.
Board of Regents of The University of Michigan
Casella Peter F.
Dautremont James F.
Teskin Robin Lyn
Wiseman Thomas G.
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