Transposon-containing DNA cloning vector and uses thereof

Details Transposon-containing DNA cloning vector and uses thereof Transposon-containing DNA cloning vector and uses thereof

1995-03-14

1997-07-08

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 4352523, 43525233, 4353201, C12N 1500

Patent

active

056459912

ABSTRACT:
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

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