Transposon consisting of a fragment of the R plasmid pCxM82B, te

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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43525232, 4353201, 536 232, C12N 121, C12N 1552, C12N 1563, C12N 1577

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active

055166695

ABSTRACT:
The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.

REFERENCES:
Kono et al. (Mar. 1983), Antimicrob. Agents Chemother., vol. 23(3), pp. 506-508.
Serwold-Davis et al. (Nov. 1988), FEMS Mirobiol. Lett., vol. 56, pp. 7-14.
Trieu-Cuot et al. (Dec. 1987), FEMS Microbiol. Lett., vol. 48, pp. 289-294.
Vandeyar et al., (Aug. 1986), J. Bacteriol., vol. 167(2), pp. 530-534.

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