Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-06-07
1998-02-17
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Vector, per se
43525233, 536 232, 536 237, 536 241, C12N 121, C12N 1511, C12N 1563
Patent
active
057190550
ABSTRACT:
A novel transposen-based vector has been developed that enhances the integration of DNA into a host genome, particularly a eukaryotic genome. The novel vector has been used, for example, in the transformation of mammalian and fish cells with a gene coding for the inductive expression of the lytic peptide cecropin B, and in the production of transgenic fish containing a gene coding for the inductive expression of the lytic peptide cecropin B. This vector shows greater transformation efficiencies than do other means for transforming a gene into a eukaryotic chromosome. The novel vector allows the rapid and efficient transformation of a eukaryotic genome. Its use does not require the high level of skill needed for microinjections. Nor does it rely on homologous recombination events for a successful transformation, as do the prior methods of microinjection, electropotation, and lipofection. A novel modification of mini-transposons was made to adapt them to carry a gene of interest into a genome.
REFERENCES:
patent: 4670388 (1987-06-01), Rubin et al.
patent: 5102797 (1992-04-01), Tucker et al.
Kleckner et al., "Uses of Transposons with Emphasis on Tn10," pp. 139-180 in Miller ed.), Methods in Enzymology, vol. 204 (1991).
J. Way et al., "New Tn10 Derivatives for Transposon Mutagenesis and for Construction of lacZ Operon Fusions by Transposition," Gene, vol. 32, pp. 369-79 (1984).
Board of Supervisors of Louisiana State University and Agricultu
Nelson Amy J.
Robinson Douglas W.
Runnels John H.
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