Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1987-06-10
1989-05-16
Martinell, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435 91, 4351721, 435320, 536 27, 935 6, 935 55, 935 56, 935 57, 935 58, C12N 1500, C12P 2100, C12P 1934, C07H 2104
Patent
active
048309656
ABSTRACT:
Transposable linkers are DNA sequences recognized and employed by transposition proteins, including transposases, in the insertion of genetic information into the genetic architecture of an organism and in the movement of genetic information from one location to another in the genetic architecture of an organism. Such linkers, comprising the extreme ends of transposons, often referred to as left and right attachment sites, have been isolated from the intervening material in transposons to produce a basic building block comprising simply the extreme ends of the transposons fused together. Using restriction endonuclease recognition sites outside and between the left and right attachment sites it is possible to introduce desired genes directly or via cloning vehicles, into the genetic material of an organism. A transposable linker may also be used to introduce a desired gene, that has been placed in a cloning vehicle but is not, of itself, part of a normally transposable sequence, into the genetic material of an organism.
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patent: 4590162 (1986-05-01), Grinter
Van Gijsegem et al, "Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Kleibsiella pneumoniae, and Proteus mirabilis", Plasmid 7: 30 (1982).
Genetic Technology News, Nov. 1982, p. 6, "Transposons act as vectors to transfer DNA into fruit files".
Castilho et al, "Plasmid insertion mutagenesis and lac gene fusion with mini*mu bacteriphage transposons", J. Bacteriol. 158: 488 (1984).
Bukhari, deceased Ahmad I.
Goodchild John
Narang Saran A.
Canadian Patents and Development Ltd.
Cold Spring Harbor Laboratory
Martinell James
Thomson Alan A.
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