Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-04-19
2005-04-19
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
06881539
ABSTRACT:
A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.
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Arbuckle John A.
Bowen Benjamin A.
Fox Timothy W.
McElver John A.
Meeley Robert B.
Callistein Steve
Horlick Kenneth R.
Pioneer Hi-Bred International , Inc.
Tung Joyce
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