Transposable cloning vector

Details Transposable cloning vector Transposable cloning vector

1983-02-14

1986-05-20

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 935 29, 935 52, C12N 1500, C12N 120, C12N 100

Patent

active

045901627

ABSTRACT:
Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A), a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function. The DNA material to be inserted into the chromosome of the recipient bacterium is included in the DNA of transposon (A) carried by the first plasmid. Any suitable transposon may be used as transposon (A) but transposon Tn7 is preferred.

REFERENCES:
Rubin et al., Science vol. 218, pp. 348-353, Oct. 1982.
Calos et al., Cell vol. 20, pp. 579-595, Jul. 1980.
Sapienza et al., Cold Spring Harbor Symposia on Quantitative Biology, vol. XLV, pp. 177-182 (1981).
"Transposition of a DNA Sequence Encoding Trimethoprim and Streptomycin Resistances from R 483 to Other Replicons"--Barth, P. T., Datta N., Hedges R. W., Grinter N. J.; J. Bact., 125, 800-810, (1976).
"Transposition of DNA Inserted into Deletions of the Tn 5 Kanamycin Resistance Element"--Meyer R., Boch G., Shapiro T.; Molecular & General Genetics, 171, 7 13, (1979).

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