Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1994-09-09
1998-04-14
Davenport, Avis M.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
530324, 530326, A61K 3800, A61K 3802, C07K 500, C07K 700
Patent
active
057392730
ABSTRACT:
A water-soluble polypeptide having the characteristics of the bacteriorhodopsin C helix such that it is largely hydrophobic and spontaneously inserts into a biological membrane as an alpha-helix. The polypeptide is administered as an aqueous solution or is contained within the lipid bilayer of a liposome. The polypeptide is modified so that it will interfere with the helix--helix interactions within the membranes of adverse cells or is coupled to a therapeutic substance, such as a protein or pharmaceutical compound, for transmission thereof through the membrane and into the cell.
REFERENCES:
Popot et al, Biochemistry, vol. 29, No. 17, 1990, pp. 4031-4037.
Danshina et al., Ach. Biochem. Biophys. vol. 279, No. 2, Jun. 1990, pp. 225-231.
Bormann, B.J. and D.M. Engelman, "Intramembrane Helix-Helix Association in Oligomerization and Trasnmembrane Signaling," Annu. Rev. Biophys. Biomol. Struct. 21:223-243 (1992).
Abrams, et al., "Identification of Translocated Gating Charge in a Voltage-dependent Channel," J. Gen. Physiol. 98:77-93 (1991).
Briggs, et al., "Conformations of Signal Peptides Induced by Lipids Suggest Initial Steps in Protein Export," Science, vol. 233, pp. 206-208 (Jul. 1986).
Briggs, et al., "In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli LamB Signal Peptides," Science, vol. 288, pp. 1096-1098 (May 31, 1985).
Cornell, et al., "Conformations and Orientations of a Signal Peptide Interacting with Phospholipid Monolayers," Biochemistry, vol. 28, No. 7, pp. 2789-2797 (1989).
Engelman, et al., "The Spontaneous Insertion of Proteins into and across Membranes: The Helical Hairpin Hypothesis," Cell 23:411-422 (1981).
Engelman et al., "Identifying Nonpolar Transbilayer Helices in Amino Acid Sequences of Membrane Proteins," Ann. Rev. Biophys. Chem. 15:321-353 (1986).
Geller, et al., M13 Procoat Inserts into Liposomes in the Absence of Other Membrane Proteins, The Journal of Biological Chemistry, vol. 260, No. 24, pp. 13281-13285 (Oct. 25, 1985)
Goormaghtiah, et al., "Secondary Structure and Orientation of the Amphipathic Peptide GALA in Lipid Structures, An Infrared-Spectroscopic Approach" European Journal of Biochemistry, vol. 195, No. 1, pp. 421-429 (Jan. 1, 1991).
Hoch, et al., "Channels Formed by Botulinum, Tetanus, and Diphtheria Toxins in Planar Lipid Bilayers: Relevance to Translocation of Proteins Across Membranes," Proc. Nat. Acad. Sci. 82:1692-1696 (1985).
Hunt, et al., "Biophysical Studies of the Integral Membrane Protein Folding Pathway," Biophysical Journal Program and Abstracts, 35th Annual Meeting, Moscone Center, San Francisco, California, Abstract No. W-AM-K2 (Feb. 24-28, 1991).
McKnight, et al., "Fluorescence Analysis of Tryptophan-Containing Variants of the LamB Signal Sequence upon insertion into a Lipid Bilayer," Biochemistry, vol. 30, No. 25, pp. 6241-6246 (1991).
McKnight, et al., "Functional and Nonfunctional LamB Signal Sequence Can Be Distinguished by Their Biophysical Properties," The Journal of Biological Chemistry, vol. 264, No. 29, pp. 17293-17297 (Oct. 15, 1989).
Neville, et al., "Transmembrane Transport of Diphtheria Toxin, Related Toxins, and Colicines," Ann. Rev. Biochem. 55:195-224 (1986).
Parente, et al., "Association of pH-Sensitive Peptide with Membrane Vesicles: Rose of Amino Acid Sequence," Biochemistry, vol. 29, No. 37, pp. 8713-8719 (1990).
Parente, et al., "pH-dependent Fusion of Phosphatidylcholine Small Vesicles," The Journal of Biological Chemistry, vol. 263, No. 10, pp. 4724-4730 (Apr. 5, 1988).
Song, et al., "Membrane Topography of ColE1 Gene Products: the Hydrophobic Anchor of the Colicin E1 Channel is a Helical Hairpin," J. Bacteriol. 173:2927-2934 (1991).
Subbarao, et al., "pH-Dependent Bilayer Destabilization by an Amphipathic Peptide," Biochemistry, vol. 26, No. 11, 2964-2972 (1987).
Von Heijne, G. "Signal Sequences are not Uniformly Hydrophobic," Journal of Molecular Biology, vol. 159, No. 4, pp. 537-541 (Aug. 25, 1982).
Wickner, W., "Assembly of Proteins into Membranes," Science 210:861-868 (1980).
Wolfe, et al., "Effects of Two sec Genes on Protein Assembly into the Plasma Membrane of Escherichia coli," The Journal of Biological Chemistry, vol. 260, No. 3, pp. 1836-1841 (Feb. 10, 1985).
Engelman Donald M.
Hunt John F.
Davenport Avis M.
Yale University
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