Transmembrane cell culture device

Details Transmembrane cell culture device Transmembrane cell culture device

1994-04-12

1995-11-28

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

4352975, 4353031, 4353052, 4352873, 4352878, 4352884, C12M 306

Patent

active

054707439

ABSTRACT:
A removable cell culture insert support device is provided which allows the initial development of cell cultures on the bottom membrane surface of an inverted cell culture insert. Following successful cell culture development, the support device of the invention is discarded and the conventional insert is reoriented and then suspended in a conventional multi-well plate with the initially developed cells on the bottom surface of the insert membrane. The upper surface of the membrane is then available for developing a second cell type for transmembrane interaction as required. The removable support includes a cup-shaped chamber with a surrounding skirt to accommodate the cell insert of interest, with the removable support having appropriate cooperating attaching surfaces. A double-sided adhesive may be used to temporarily join the two parts together.

REFERENCES:
patent: 3990852 (1976-11-01), Piazzi et al.
patent: 4308351 (1981-12-01), Leighton et al.
patent: 4748124 (1988-05-01), Vogler
patent: 4871674 (1989-10-01), Matsui et al.
patent: 4959197 (1990-09-01), Parekh et al.
patent: 5026649 (1991-06-01), Lyman et al.
"Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins" In Vitro Cell Dev. Biol. 26:1135-1143, Dec. 1990 1990 Tissue Culture Association James B. Mangum, Jeffrey I. Everitt, James C. Bonner, Lynne R. Moore and Arnold R. Brody.

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