Translational elongation factor promoter from Pichia...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S320100, C435S254200, C435S325000

Reexamination Certificate

active

07816509

ABSTRACT:
Disclosed are aPichia pastoris-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express thePichia pastoris-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.

REFERENCES:
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patent: 5650294 (1997-07-01), Kurth et al.
patent: 6011147 (2000-01-01), Nakari et al.
patent: 6265185 (2001-07-01), Muller et al.
CL 489166, disclosed in reference Sessions et al. Plant Cell, 2002. vol. 14, No. 12, pp. 2985-2994.
Muller, Yeast, vol. 14, Issue 14, pp. 1267-1283, John Wiley & Sons, Ltd., Published Online Dec. 4, 1998.
International Search Report for PCT/KR2006/000215, mailed Aug. 10, 2006.
Written Opinion of the International Searching Authority for PCT/KR2006/000215, mailed Aug. 10, 2006.

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