Translation of m-RNA

Details Translation of m-RNA Translation of m-RNA

1993-04-12

1996-02-06

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352523, 4352402, 4352404, 43525233, 4353201, 536 241, 935 34, 935 35, 935 44, 935 66, C12N 510, C12N 121, C12N 1567, C12N 1511

Patent

active

054895278

ABSTRACT:
It has been found that the 5' regions of RNA viruses act as enhancers of translation of mRNA. The omega prime (.OMEGA.') sequence of Tobacco Mosaic Virus, derivatives and portions thereof, and 5' leader sequences of other RNA viruses can be ligated upstream of an appropriate mRNA or used in the form of cDNA in an expression vector. The translational enhancers are effective in many different types of expression system, for example plant cells, bacteria, and animal cells, and also in in vitro translation.

REFERENCES:
patent: 4820639 (1989-04-01), Gehrke
patent: 4962028 (1990-10-01), Bedbrook et al.
Parks, G. D. et al. 1986. J. Virology vol. 60 p. 376-384.
Wilson, T. M. A. 1986. "Expression of the Large 5' Proximal Cistron of Tobacoo Mosaic Virus by 70s Ribosomes During Cotranslational Disassembly in a Prokaryotic Cell-Free System." Virology vol. 152 pp. 277-279.
Sleat, D. E., et al, 1987. "Characterization of the 5'-leader Sequence of Tobacco Mosaic Virus RNA as a General Enhancer of Translation in vitro". Gene vol. 17 pp. 217-225.
Science, vol. 231, 14 Mar. 1986, R. French et al.: "Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyle-donous plant cells", pp. 1294-1297.
Chemical Abstracts, vol. 103, 1985 (Columbus, Ohio, US) L. Sue Loesche-Fries et al.: "Expression of alfalfa mosaic virus RNA 4 cDNA transcripts in vitro and in vivo", see Abstract No. 190686.
Proc. Natl. Acad. Sci., vol. 83, Mar. 1986, (US) W. O. Dawson et al.: "cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts", pp. 1832-1836.
Nucleic Acids Research, vol. 15, No. 8, 24-Apr.-1987 D. R. Gallie et al.: "The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo", pp. 3257-3273.
Proc. Natl. Acad. Sci. (USA), vol. 79, Oct. 1982, P. Goelet et al.: "Nucleotide sequence of tobacco mosaic virus RNA", pp. 5818-5822.
Biological Abstracts, vol. 82, No. 5, 1986 (Philadelphia, PA, US) L. Gehrke: "Preparation of messenger RNA transcripts for secondary structure analysis using SP6 polymerase, guanylyltransferase and preparative gel electrophoresis" see abstract No. 40900.
Chemical Abstracts, vol. 88, 1978 (Columbus, Ohio, US) K. Richards et al.: "Nucleotide sequence at the 5' extremity of tobacco mosaic virus RNA. 1. The noncoding region (nucleotides 1-68)", see p. 212, abstract No. 165637t.
Nature, vol. 316, 15-Aug.-1985 T. Hunt: "False starts in translational control of gene expression"pp. 580-581.
Biochemistry, vol. 22, 1983, American Chemical Society; L. Gehrke et al.: "5'-conformation of capped alfalfa mosaic virus ribonucleic acid 4 may reflect its independence of the cap structure or of cap-binding protein for efficient translation", pp. 5157-5164.
Chemical Abstracts, vol. 89, 1978 (Columbus, Ohio, US) W. Zagorski: "Translation regulation of expression of the brome-mosaic-virus RNA genome in vitro", see p. 213, abstract no. 72850n.
Nature, vol. 313, 28-Feb.-1985 J. T. Odell et al.: "Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter", pp. 810-812.
The EMBO Journal, vol. 4, No. 8, 1985; M. W. Bevan et al.: "Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium", pp. 1921-1926.
Loesch-Fries et al. Virology, vol. 146, pp. 177-187 (1985).
Gehrke, L, Gene Anal. Tech 3(3) pp. 45-52, (1986).
Richard et al, European J. Biochemistry, 84(2), pp. 513-519 (1978).
W. Zagorski, European Journal of Biochemistry, 86(2), pp. 465-472 (1978).
Darlix et al., Nucleic Acids Res., 10(17), pp. 5183-5196 (1982).
Filipowicz & Haenni, Proc. Nat. Acad. Sci. (USA), vol. 76(7), pp. 3111-3115, (1979).
A Dictionary of Genetic Engineering (S. G. Oliver & J. M. Ward), Cambridge University Press, 1985, p. 35.
Mundry 2, Vererbungsl, 97, 281 (1965).
Mandeles, Journal of Biological Chemistry, vol. 243 (13), pp. 3671-3674 (1968).
Konarska et al., Eur. J. Biochem., vol. 114, pp. 221-227 (1981).
Wilson et al., J. Gen. Virol., vol. 66, pp. 1201-1207 (1985).

Affiliated with

Also associated with

Rating

Translation of m-RNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Translation of m-RNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Translation of m-RNA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2174606