Transglycosylation method and protein having...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S085000, C435S101000, C435S252300, C435S320100

Reexamination Certificate

active

07445921

ABSTRACT:
The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of a protein having an endo-α-N-acetylgalactosaminidase activity and a transglycosylation activity.

REFERENCES:
patent: 6740509 (2004-05-01), Karakasa et al.
patent: 1 227 152 (2002-07-01), None
patent: 01/42215 (2001-06-01), None
patent: 01-42225 (2001-06-01), None
Bardales et al., Transglycosylation and transfer reaction activities of Endo-alpha-N-acetyl-D-galctosaminidase from Diplococcus (Streptococcus) pneumoniae. J.Biol.Chem., 1989, vol. 33 (25): 19893-19897.
Ashida et al., Characterization of Endo-alpha-N-acetylgalactosaminidase fromBacillussp., and synthesis of Neo-oligosaccharides using its transglycosylation activity. Arch. Biochem Biophysics., 2000, vol. 373 (2): 394-400.
Schell et al., The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc. Nat. Acad. Sci., 2002, vol. 99 (22): 14422-14427.
Oura et a., Molecular cloning and characterization of endo-aplha-N-acetylgalactosaminidase from Bifidobacterium longum. Abstract 466, Joint Meeting of the Society for Glycobiology and Japanese Society of Carbohydrate Research, Nov. 17-20, 2004, Honolulu, HI, USA.
Whisstock et al., Prediction of protein function from protein sequence and structure. Q Rev Biophysics., 2003, vol. 36 (3): 307-340.
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T. Katayama, et al., “Novel Bifidobacterial Glycosidases Acting on Sugar Chains of Mucin Glycoproteins”, Journal of Bioscience and Bioengineering, vol. 99, No. 5, 2005, pp. 457-465, XP-004997036.
K. Fujita, et al., “Identification and Molecular Cloning of a Novel Glycoside Hydrolase Family of Core 1 Type O-Glycan-Specific Endo-Alpha-Acetylgalactosaminidase From Bifidobacterium Longum”, The Journal of Biological Chemistry, vol. 280, No. 45, Nov. 11, 2005, pp. 37415-37422, XP-002383896.
Dostal, V., et al., “Determination of Acids and Their Strenth in Isobutyl Methyl Ketone,” Colleciton of Czechoslovak Chemical Communications, vol. 47, No. 4, 1982, pp. 1203-1215, XP002405365.
Certificate by the Chairperson of Japan Society for Bioscience, Biotechnology and Agrochemistry, including documents such as a abstract of the presentation of Mar. 30, 2004 by Oura et al, and partial translations thereof.
Certificate by the Chairperson of Japanese Society of Carbohydrate Research, including documents such as a abstract of the presentation of May 29, 2004 by Oura et al., and partial translations thereof.

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