Transgenic trees exhibiting increased growth, biomass...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S290000, C800S278000, C800S287000, C435S419000

Reexamination Certificate

active

07807878

ABSTRACT:
Important aims in nearly all tree-breeding programs around the world are to produce plants with increased growth rates and stem volumes, and shorter rotation times. Such trees would yield more biomass per area unit. Here the present inventors have shown that when over-expressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hybrid aspen (Populus tremula×P. tremuloides), improvements in valuable traits such as growth rate and biomass are obtained. In addition, these trees also have longer xylem fibres than unmodified wild type plants. Long fibers are very desirable in the production of strong paper, but it has not (as yet) proved possible to influence this trait by traditional breeding techniques. A further advantage of the present invention is that it may reduce or eliminate the use of growth influencing chemicals in forestry.

REFERENCES:
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Huang, S. et al. (1998). “Overexpression of 20-Oxidase Confers a Gibberellin-Overproduction Phenotype in Arabidopsis,” Plant Physiology. 118:773-781.
Fladung, M. (Sep. 22-25, 1999) “Transgenic Trees for a Better World?,” Proceedings of the Internal Congress-Application of Biotechnology to Forest Genetics BioFor '99, Vitoria-Gasteiz, Spain, pp. 339-345.
Coles, J. P. et al. (1999). “Modification of Gibberellin Production and Plant Development in Arabidopsis by Sense and Antisense Expression of Gibberellin 20-Oxidase Genes,” The Plant Journal 17(5): 547-556.
Ellis, D. et al. (2001) “Transgenic Trees: Where are We Now?,” Proceedings of the First International Symposium on Ecological and Societal Aspects of Transgenic Plantations, Oregon State University, 2001, pp. 113-123.
Eriksson, M. E. et al. (2000). “Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length,”Nature Biotechnology18:784-788.

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