Transgenic trees exhibiting increased growth, biomass...

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...

Reexamination Certificate

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C800S260000

Reexamination Certificate

active

07141422

ABSTRACT:
Important aims in nearly all tree-breeding programs around the world are to produce plants with increased growth rates and stem volumes, and shorter rotation times. Such trees would yield more biomass per area unit. Here the present inventors have shown that when over-expressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hybrid aspen (Populus tremula×P. tremuloides), improvements in valuable traits such as growth rate and biomass are obtained. In addition, these trees also have longer xylem fibers than unmodified wild type plants. Long fibres are very desirable in the production of strong paper, but it has not (as yet) proved possible to influence this trait by traditional breeding techniques. A further advantage of the present invention is that it may reduce or eliminate the use of growth influencing chemicals in forestry.

REFERENCES:
patent: WO 94/28141 (1994-12-01), None
Walden et al (1995, TIBTECH 13:324-331).
Ahuja et al (1996, M.R. Ahuja et al (eds.) Somatic Cell Genetics and Molecular Genetics of Trees, Kluwer Academic Publishers. Netherlands. pp. 89-96).
Coles, Jeremy P. et al. (1999) “Modification of gibberellin production and plant development inArabidopsisby sense and antisense expression of gibberellin 20-oxidase genes”The Plant Journal17(5): 547-556.
Eriksson, Maria E. (Jul. 2000) “Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length”Nature Biotechnology18: 784-788.
Huang, Shihshieh et al. (1998) “Overexpression of 20-Oxidase Confers a Gibberellin-Overproduction Phenotype in Arabidopsis”Plant Physiol.118: 773-781.
Fladung, M. (Sep. 1999) “Transgenic Trees for a Better World?,” Proc. of the Intern. Congress “Application of Biotechnology to Forest Genetics” (Biofor-99), Vitoria-Gasteiz, Spain, Sep. 22-25, 1999, pp. 339-345.
Ellis, Dave et al. (2001) “Transgenic Trees: Where are We Now?,” Proceedings of the First International Symposium on Ecological and Societal Aspects of Transgenic Plantations, Oregon State University, 2001, pp. 113-123.

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