Transgenic tomato plants with altered polygalacturonase isoforms

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800255, 800DIG44, 4351723, 4353201, 4352523, 4352404, A01H 400, C12N 121, C12N 514, C12N 1582

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active

055698310

ABSTRACT:
Methods of creating transgenic tomatoes containing a lowered level of polygalacturonase isoform 1 are disclosed. The isolation of a DNA sequence encoding the polygalacturonase beta-subunit is disclosed. The beta-subunit sequence can be used to construct both sense and antisense plant expression constructions which can be transformed into tomato plants. The transgenic tomato plants have altered levels of polygalacturonase isoforms in that the level of isoform 1 is dramatically reduced. The resulting tomato fruit has a polygalacturonase activity level that is more heat labile, and thus more convenient for processing, and an increased level of soluble pectins.

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