Transgenic swine compositions and methods

Details Transgenic swine compositions and methods Transgenic swine compositions and methods

1995-06-06

1999-08-24

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4351723, 435378, C12N 500, C12N 1500

Patent

active

059424354

ABSTRACT:
Transgenic swine, and compositions and methods for making and using same, are provided. Central to the invention are porcine (Sus scrofa) embryonic stem cell lines and methods for establishing them. Cells of such lines are transformed with exogenous genetic material of interest and then used to provide chimeric swine, which have germ cells comprising the exogenous genetic material. The chimeric swine are bred to provide transgenic swine. Transgenic swine of the invention can be used to provide human proteins or peptide hormones or can be used as xenograft donors.

REFERENCES:
patent: 5175383 (1992-12-01), Leder et al.
patent: 5175384 (1992-12-01), Krimpenfort et al.
patent: 5175385 (1992-12-01), Wagner et al.
patent: 5523226 (1996-06-01), Wheeler
patent: 5690926 (1997-11-01), Hogan
Axelrod, H.R., "Embryonic Stem Cell Lines Derived from Blastocysts by a Simplified Technique," Developmental Biology, 101:225-228 (1984).
Bazer, F.W., et al., "Fertilization, Cleavage and Implantation," in Reproduction in Farm Animals, 5th ed., ed. Hafez, E.S.E., Lea & Febiger, Philadelphia, PA (1987).
Bearden, J.H., et al., "Gestation," Applied Animial Reproduction, pp. 85-98, Reston Publishing Company, Reston, VA (1980).
Bishop, Jerry E., Sheep-Cloning Method Holds Promise Of Fast Introduction of Livestock Traits, News Article, 1996.
Bleck, G.T., et al., "Single-Base Polymorphisms and DNA Sequence of the Porcine .alpha.-Lactalbumin 5'Flanking Region," J. Dairy Sci., (Suppl.1):946 (1994).
Bradley, A., et al., "Formation of germline chimeras from embryo-derived teratocarcinoma cells lines," Nature, 309:255-256 (1984).
Brinster, et al., "Targeted correction of a mjaor histocompatibility class II E.alpha. gene by DNA microinjected into mouse eggs," Proc. Natl. Acad. Sci., 86:7087-7091 (1989).
Capecchi, Mario R., "The new mouse genetics: Altering the genome by gene targeting," Trends in Genetics, 5(3):70-76 (1989).
Clark, A.J., et al., "Germ line manipulation: applications in agriculture and biotechnology," Transgenic Animals, p. 250, Grosveld, et al., eds., Academic Press Limited (1992).
Clark, et al., "Expression of human anti-hemophilic factor IX in the milk of transgenic sheep," Biotechnology, vol. 7:487-492 (1989).
Cruz, Y.P., et al., "Origin of embryonic and extraembryonic cell linages in mammalian embryos," in Animal Application of Research in Mammalian Development, eds. Pedersen, R.A., et al., Cold Spring Harbor Laboratory Press, Plainview, NY (1991).
Deotschman, T., et al., "Establishment of Hamster Blastocyst-Derived Embryonic Stem (ES) Cells," Developmental Biology, 127:224-227 (1988).
Ebert, K.M., et al., "Changes in Domestic Livestock through Genetic Engineering," in Current Communications 4 Cell & Molecular Biology, Animal Applications of Research in Mammalian Development, ed. Pederson et al., Cold Springs Harbor Laboratory Press, pp. 233-266 (1991).
Evans, M.J., et al., "Establishment in culture of pluripotential cells from mouse embryos," Nature, 292:154-156 (1981).
Fajfar-Whetstone, C.F., et al., "Sex Determination of Porcine Pre-Implantation Embryos via Y-Chromosome Specific DNA Sequence," Anim. Biotech., 4:183-193 (1993).
Flake, A.W., et al., "Transplantation of fetal hematopoietic stem cells in utero: the creation of hematopoietic chimeras," Science, vol. 233 (1986).
Frohman and Martin, "Cut, paste, and save: New approaches to altering specific genes in mice," Cell, 56:145-147 (1989).
Gossler, A., et al., "Transgenesis by means of blastocyst-derived embryonic stem cell lines," Proc. Natl. Acad. Sci. USA, 83:9065-9069 (1986).
Hagen, D.R., et al., J. Anim. Sci., 69:1147-1150 (1991).
Handyside, A., et al., "Towards the Isolation of Embryonal Stem Cell Lines from the Sheep," Roux's Arch, Developmental Biology, 196:185-190 (1987).
Hasty, et al., "The length of homology required for gene targeting in embryonic stem cells," Molecular and Cellular Biology, 11(11):5586-5591 (1991).
Hogan, et al., "Isolation of Pluripotential Stem Cell Lines," Cold Spring Harbor Laboratory--Manipulating the Mouse Embryo: A Laboratory Manual, Section E:205-218 (1986).
Hooper, M.C., Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, ed. Robertson, E.J., IRL Press, Ltd., Oxford, UK, 51-70 (1987).
Jasin and Berg, "Homologous integration in mammalian cells without target gene selection," Genes & Development, 2:1353-1363 (1988).
Jeannotte, et al., "Low level of Hox1.3 gene expression does not preclude the use of promoterless vectors to generate a targeted gene disruption," Molecular and Cellular Biology, 11(11):5578-5585.
Karg, H., "Manipulation of Lactation," in Biotechnology for Livestock Production, Plenum Press, New York and London, 19:181-206 (1989).
Karg, H., "Manipulation of Growth," in Biotechnology for Livestock Production, Plenum Press, New York and London, 18:159-180 (1989).
Kollias, G., et al., "The study of gene regulation in transgenic mice," Transgenic Animals, p. 92, Grosveld, et al., eds., Academic Press Limited (1992).
Lewin, H.A., et al., "Mapping Genes for Resistance to Infectious Diseases in Animals," in Gene-Mapping Techniques and Applications, Marcel Dekker, Inc., 13:283-304 (1991).
Mansour, et al., "Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: A general strategy for targeting mutations to non-selectable genes," Nature, 336:348-352.
Martin, G.R., "Teratocarcinomas and Mammalian Embryogenesis," Science, 209:768-776 (1980).
Martin, G.R., "Isolation of a Pluripotent Cell Line from Early Mouse Embryos Cultured in Medium Conditioned by Teratocarcinoma Stem Cells," Proc. Natl. Sci. USA, 78(12):7634-7638 (1981).
Martin, P., et al., "Improvement of milk protein quality by gene technology," Livestock Production Science, 35:95-115 (1993).
McLaren A., et al., Nature, 224, pp. 238-240 (1969).
Mintz, B., Science, 148, 1232-3 (1965).
Morgenstern and Land, Nucleic Acids Res., 18:3587-3596 (1990).
Mortensen, et al., "Production of homozygous mutant ES cells with a single targeting construct," Molecular and Cellular Biology, 12(5):2391-2395 (1992).
Nichols, J., et al., "Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity," Development, 110:1341-1348 (1990).
Notarianni, E., et al., "Maintenance and differentiation in culture of pluripotential embryonic cell lines from pig blastocysts," J. Reprod. Fert., Suppl. 41:51-56 (1990).
Papaioannou, V.E., et al., "Growth and differentiation of an embryonal carcinoma cell line (C145b)," J. Embryol. exp. Morph., 54, pp. 277-295 (1979).
Phillips, R.W., et al., Models, pp. 437-440 in Swine in Biomecial Research, ed. Tumbleson, M.E., vol. 1 (Plenum Press, New York), (1986).
Piedrahita, J.A., et al., "Influence of Feeder Layer Type on the Efficiency of Isolation of Porcine Embryo-Derived Cell Lines", Theriogenology, 34(5):865-877 (1990).
Piedrahita, J.A., et al., "On the Isolation of Embryonic Stem Cells: Comparative Behavior of Murine, Porcine and Ovine Embryos," Theriogenology, 34(5):879-901 (1990).
Polge, C., "Embryo transplantation and preservation," pp. 277-291 in Control of Pig Reproduction, Cole, D.J.A., et al., eds., Butterworth Scientific, London (1982).
Robertson, E., et al., "Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector," Nature, 323:445-448 (1986).
Robertson, E.J., "Pluripotential stem cell lines as a route into the mouse germ line," Trends Genet., 2:9-13 (1987).
Robertson, E.J., "Embryo-derived Stem Cell Lines," Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, Chap. 4(4.4.2):92, ed. Robertson, E.J., IRL Press (1987).
Rohrer, G.A., Alexander, L.J., Keele, J.W., Smith, T.P., Beattie, C.W., "A Microsatellite Linkage Map of the Porcine Genome," Genetics, 136:231-245 (1994).
Rossant, J., et al., "The developmental potential of a euploid male teratocarcinoma cell line after blastocyst injection," J. Embryol. exp. Morph., 70, 99-112 (1982).
Rossant, J., et al., "The relationship between embryonic, embryonal carcinoma and embryo-derived stem cells," Cell Differentiation, 15:155-161 (1984).
Rudnicki, M.A., e

Affiliated with

Also associated with

Rating

Transgenic swine compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic swine compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic swine compositions and methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-466820