Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1994-03-10
1996-08-13
Chambers, Jasemine C.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
4351723, 435 694, 935 63, 800DIG1, C12N 500, C12N 1500, C12P 2106
Patent
active
055458084
ABSTRACT:
An "all fish" chimeric gene construct suitable for gene transfer for commercially important fish comprises the antifreeze gene (AFP) promoter fused to the desired gene sequence which is incorporated into fish embryos. The desired gene sequence is expressed in the transfected fish to provide a transgenic fish having the characteristics of the gene sequence.
REFERENCES:
Vielkind et al., The Induction of a Specific Pigment Cell Type by Total Genomic DNA Injected into the Neural Crest Region of Fish Embryos of the Genus Xiphophorus 1982, pp. 379-389.
Zhu et al., Novel gene transfer into the fertilized eggs of gold fish (Carassius auratus L. 1758), 1985.
Sekine et al., Cloning and expression of cDNA for salmon growth hormone in Escherichia coli, 1985.
Chen et al., Gene Transfer, Expression and Inheritance of Rainbow Trout and Human Growth Hormone Genes in Carp and Loach, Transgenic Models in Medicine and Agriculture, pp. 127-139, 1990, Wiley Liss, Inc.
Hew et al (1988) J. Biol. Chem. 263, 12049-12055.
Idler et al (1989) Gen. Comp. Endocrin. 74, 327-334.
Fletcher Garth L.
Hew Choy L.
Chambers Jasemine C.
Crouch Deborah
HSC Research and Development Limited Partnership
Seabright Corporation
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