Transgenic rice plants with reduced expression of Os2AP and...

Details Transgenic rice plants with reduced expression of Os2AP and... Transgenic rice plants with reduced expression of Os2AP and...

2008-01-15

2008-01-15

Kubelik, Anne (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide encodes an inhibitory rna molecule

C800S286000, C435S468000

Reexamination Certificate

active

07319181

ABSTRACT:
The aromatic compound 2-acetyl-1-pyrroline-is the major potent flavor component of all aromatic rice. This present invention provides transgenic rice plants in which 2-acetyl-1-pyrroline is synthesized at a level greater than in naturally occurring non-aromatic varieties. The transgenic plants have reduced expression of the Os2AP gene and protein, resulting in an aromatic phenotype.

REFERENCES:
patent: 5702933 (1997-12-01), Klee et al.
patent: 2006/0123505 (2006-06-01), Kikuchi et al.
Asayama M.Oryza sativa(japonica cultivar-group) BADH2 mRNA for betaine aldehyde dehydrogenase, complete cds. (2003) GenBank Accession # AB096083.
Bradbury LMT, et al. The gene for fragrance in rice. (2005) Plant Biotechnology Journal, vol. 3, pp. 363-370.
Nomenclature Committee of the INternational Union of Blochemistry and Molecular Biology (NC-IUBMB) “Classification and nomenclature of enzymes by the reactions they catalyse”; World Wide Web.chem.qmul.ac.uk/iubmb/enzymes/rules.html, pp. 1-15.
Horiguchi G. RNA silencing in plants: a shortcut to functional analysis. (2004) Differentiation, vol. 72, pp. 65-73.
Elomaa P. Transformation of antisense constructs of the chalcone synthase gene superfamily intoGerbera hybrida: differential effect on the expression of family members; (1996) Molecular Breeding, vol. 2, pp. 41-50.
Lorieux M. et al. Aroma in rice: genetic analysis of a quantitative trait. (1996) Theor Appl Genet., vol. 93, pp. 1145-1151.
Wanchana et al. Sequence Variation of BADH is Associated with the Synthesis of 2AP, a Ptent Aroma Determination in rice. (2003) Proceedings of the Conference on Rice Biotechnology 2003, Peach, Pattaya, Thailand, pp. 157-160.
Wanchana et al. Enhancing 2-acetyl-1-pyrroline synthesis in rice leaves by RNAi-mediated suppression of Os2AP converts non-aromatic to aromatic rice (Oryza sativa L.) (2004) Proceedings of the 1stInternational Conference on Rice for the Future, p. 105.
Colliver SP, Morris P, and Robbins MP. Differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in transgenicLotus corniculatus. (1997) Plant Molecular Biology, vol. 35, pp. 509-522.
Bradbury, Louis M.T. et al. (2005) “The gene for fragrance in rice” Plant Biotechnology Journal 3: 363-370.
Christopher, Mandy et al. (Aug. 2004) “Marker assisted selection in rice improvement” Rural Industries Research and Development Corporation: 15 pages.
Elsley, Kevin (Nov. 18, 2004) “Fragrant gene found” The Land: 53.
Garland, Stephen and Robert Henry (May 2001) “Application of Molecular Markers to Rice Breeding in Australia” Rural Industries Research and Development Corporation: 21 pages.
Kamolsukyunyong, W. et al. (Jul. 18, 2003) “Isogenic Lines Carrying the 26.7-kb Genomic Region on Chromosome 8 of KDML105 are Characterized as Aromatic Rice,” Proceeding of the Conference on Rice Biotechnology, Peach, Pattaya, Thailand, pp. 151-155.
Kamolsukyunyong, Wintai et al. (2003) “Aroma Gene of Thai Hom Mali Rice,” Lab Today, 9:66-69.
Nagsuk, Anujaree et al. (2003) “Identification of 2-Acetyl-1-Pyrroline, the Principal Aromatic Rice Flavor Compound, in Fungus Cultures,” Proceedings of the 2nd International Conference on Medicinal Mushrooms & International Conference on Biodiversity and Bioactive Compounds, pp. 395-400.
Vanavichit, A. et al. (Aug. 31-Sep. 3, 2004) “Discovering Genes for Rice Grain Aroma,” Proceedings of the 1st International Conference on Rice for the Future, Bangkok, Thailand, pp. 71-80.
Wanchana, S. et al. (Jul. 18, 2003) “Sequence Variation in BADH is Associated with Synthesis of 2AP, a Potent Aroma Determination in Rice,” Proceedings of the Conference on Rice Biotechnology 2003, Peach, Pattaya, Thailand, pp. 157-160.
Wanchana, Samart et al. (2001) “Physical Mapping of the Region Proximal to Genes Controlling Aroma in Rice,” RGJ-Ph.D. Congress II, Chonburi, Thailand, p. 153.
Wanchana, Samart et al. (2003) “Sequence Variation in BADH is Associated with the Synthesis of 2AP, a Potent Aroma Determination in Rice,” RGJ-PH.D. Congress IV, Chonburi, Thailand, p. 162.
Wanchana, Samart et al. (2005) “RNAi-Mediated Suppression of Os2AP Converts Non-aromatic to Aromatic Rice,” RGJ-PH.D. Congress VI, Chonburi, Thailand, p. 160.
Wanchana, Samart et al. (Aug. 10-11, 2004) “Sequence Variation on BADH Associated with 2-Acetyl-1-Pyrroline, the Potent Aroma Compound in Rice,” The 4th National Symposium on Graduate Research, Lotus Hotel Pang Suan Kaew, Chiang Mai, Thailand, p. 105.
Wanchana, Samart et al. (Aug. 31-Sep. 3, 2004) “Enhancing 2-Acetyl-1-Pyrroline Synthesis in rice Leaves by RNAi-Mediated Suppression of Os2AP Converts Non-aromatic to Aromatic Rice (Oryza sativa L.),” Proceedings of the 1st International Conference on Rice for the Future, Bangkok, Thailand, p. 105.
Wanchana, Samart et al. (2005) “A Rapid Construction of a Physical Contig across a 4.5 cM Region for Rice Grain Aroma Facilitates Marker Enrichment for Positional Cloning,” ScienceAsia, 31: 299-306.

Affiliated with

Also associated with

Rating

Transgenic rice plants with reduced expression of Os2AP and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic rice plants with reduced expression of Os2AP and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic rice plants with reduced expression of Os2AP and... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2774726