Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters plant part growth
Reexamination Certificate
2005-06-28
2005-06-28
Nelson, Amy J. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters plant part growth
C800S278000, C800S294000, C800S295000, C800S298000, C536S023100, C536S023200, C536S023600, C435S320100, C435S419000
Reexamination Certificate
active
06911576
ABSTRACT:
Nitrogen is one of the principal factors limiting vegetative production. The present invention has improved the nitrogen metabolism in Poplar by integrating a transgene constitutively expressing a pine glutamine synthetase into the plan genome. The resulting transgenic trees exhibit higher growth rates, protein and chlorophyll contents, and leaf area than equivalent untransformed trees. It is contemplated that this approach to nitrogen improvement will be equally successful for all woody perennials. Provided with the invention is an expression casette, a vector, and a method for increase glutamine synthetase activity in woody perennials, as well as transgenic woody perennials with enhanced nitrogen metabolism and accompanying phenotype.
REFERENCES:
patent: 5955651 (1999-09-01), Coruzzi
patent: 0303780 (1988-05-01), None
Feuillet et al., Tissue-and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants. 1995, Plant Molecular Biology, vol. 27, pp. 651-667.
Canton et al., Molecular charaterization of a cDNA clone encoding glutamine synthetase from a gymnosperm,Pinus sylvestris.1993, Plant Molecular Biology, vol. 22, pp. 819-828.
van Engelen et al., pBINPLUS: an improved plant transformation vector based on pBIN19. 1995. Transgenic Research, vol. 4, pp. 288-290.
Noir et al., Origin, diversity and evolution of NBS-type disease-resistance gene homologues in coffee tree (Coffea L). 2001. Mol Genet Genomics, vol. 265, pp. 654-662.
Genbannk Accession No. X69822.
Fourgoux-Nicol et al., 1999, Plant Molecular Biology, vol. 40 :857-872.
Alba Fernardo Gallardo
Kirby Edward G.
Ramos Francisco Canovas
Dann Dorfman Herrell & Skillman
Kallis Russell
Nelson Amy J.
Rutgers The State University of New Jersey
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