Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-09-22
1999-12-07
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
4352522, 4352523, 4353201, 435419, 536 232, A01H 500, A01H 510, C07H 2104, C12N 121
Patent
active
059987022
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
This invention relates to the plant enzyme ACC synthase which is essential for the production of ethylene in higher plants. More particularly, the invention relates to the DNA sequence of a Brassica oleracea ACC synthase, DNA constructs containing this sequence, plant cells containing the constructs and plants derived therefrom.
BACKGROUND OF THE INVENTION
The enzyme ACC synthase is essential to the production of ethylene in higher plants. It is well known that ethylene is related to various events in plant growth and development including fruit ripening, seed germination, abscission, and leaf and flower senescence. Ethylene production is strictly regulated by the plant and is induced by a variety of external factors known as ethylene-inducible events. These include the application of auxins, wounding, anaerobic conditions, viral infection, elicitor treatment, chilling, drought, and exposure to ions such as cadmium and lithium ions. In addition, it recently has been shown that ethylene production begins after harvest (Tian et al., J. Amer. Soc. Hort. Sci., 119:276-281 (1994)).
The pathway for ethylene synthesis in plants was first described by Adams and Yang, PNAS, U.S.A., 76:170-174 (1979) who identified 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. The physiology and biochemistry of ethylene synthesis was extensively reviewed by Yang and Hoffman in Ann. Rev. Plant Physiol., 35:155-189 (1984). In the ethylene biosynthetic pathway, methionine is catalyzed by the enzyme S-adenosylmethionine synthetase to form S-adenosylmethionine (SAM). SAM is then converted to 1-aminocyclopropane-1-carboxylic acid (ACC) by the enzyme ACC synthase. This three-membered-ring amino acid is then metabolized to yield ethylene, a reaction catalyzed by the enzyme ACC oxidase.
The ethylene-forming enzyme genes in tomato were the first to be isolated. Smith et al., Planta, 168:94-100 (1986) reported the rapid appearance of an mRNA correlated with ethylene synthesis, which encodes a protein of molecular weight 35,000. This formed the basis for the development of a number of molecular strategies to inhibit ethylene formation in certain transgenic plants. One such method is based on antisense RNA.
As is well known, a cell manufactures protein by transcribing the DNA of the gene encoding that protein to produce RNA, which is then processed to messenger RNA (mRNA) (e.g., by the removal of introns) and finally translated by ribosomes into protein. This process may be inhibited by the presence in the cell of "antisense RNA." The term antisense RNA means an RNA sequence which is complementary to a sequence of bases in the mRNA in question in the sense that each base (or the majority of bases) in the antisense sequence (read in the 3' to 5' sense) is capable of pairing with the corresponding base (G with C, A with U) in the mRNA sequence read in the 5' to 3' sense. It is believed that this inhibition takes place by formation of a complex between the two complementary strands of RNA, thus preventing the formation of protein. How this works is uncertain: the complex may interfere with further transcription, processing, transport or translation, or degrade the mRNA, or have more than one of these effects. This antisense RNA may be produced in the cell by transformation of the cell with an appropriate DNA construct arranged to transcribe the non-template strand (as opposed to the template strand) of the relevant gene (or of a DNA sequence showing substantial homology therewith).
The use of anti-sense RNA to downregulate the expression of specific plant genes is well known. Reduction of gene expression has led to a change in the phenotype of the plant: either at the level of gross visible phenotypic difference, e.g., lack of anthocyanin production in flower petals of petunia leading to colorless instead of colored petals (van der Krol et al., Nature, 333:866-869 (1988)); or at a more subtle biochemical level, e.g., change in the amount of polygalacturonase and redu
REFERENCES:
patent: 5365015 (1994-11-01), Grierson et al.
patent: 5512466 (1996-04-01), Klee et al.
patent: 5702933 (1997-12-01), Klee et al.
Napoli et al. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. The Plant Cell. 2:279-289, Apr. 1990.
Wen et al. Nucleotide sequence of a cDNA clone encoding 1-aminocyclopropane-1-carboxylate synthase in mustard (Brassica juncea [L.] Czern & Coss). Plant Physiology. 103:1019-1020, 1993.
Smith et al. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature. 34:724-726, Aug. 1988.
David et al. Genetic transformation of cauliflower (Brassica oleracea L. var. Botrytis) by Agrobacterium rhizogenes. Plant Cell Reports. 7:88-91, 1988.
Wagoner, Wendy J., et al., HortScience Abstract 348, 27:(6),620-621 (1992).
EMBL ACC., No. X82273, Rel. 41.(31-10-1994).
EMBL No. X72676, (May 1994).
Clarke, Sean F., et al., The influence of 6-benzylaminopurine on post-harvest senescence of floral tissues of broccoli (Brassica oleracea var Italica), Plant Growth Regulation, 14:21-27, (1994).
Anderson, E.J. et al., Transgenic Plants That Express the Coat Protein Genes of Tobacco Mosaic Virus of Alfalfa Mosaic Virus Interfere with Disease Development of Some Nonrelated Viruses, The American Phytopathological Society, 79(11);1284-1289, (1989).
Hampilton, A.J. et al., Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast, Proc. Natl. Acad. Sci. USA, 88:7434-7437, (1991).
Kim, Woo Taek, et al., Structure and expression of cDNA As encoding 1-aminocyclopropane-1-carboxylate oxidase homologs isolated from excised mung bean hypocotyls, Planta, 194:223-229, (1994).
Larsen, Paul B., et al., Cloning and Nucleotide Sequence of a S-Adenosylmethionin Synthetase cDNA from Carnation.sup.1, Plant Physiol, 96:997-999, (1991).
Lawton, Kay A., et al., Molecular Cloning and Characterization of Senescence-Related Genes from Carnation Flower Petals.sup.1, Plant Physiol. 90:690-696, (1989).
Gomez-Lim, Miguel Angel, et al.,Isolation and characterization of a gene involved in ethylene biosynthesis from Arbidopsis thaliana, Gene, 134:217-221, (1993).
Klee, Harry J., Ripening Physiology of Fruit from Transgenic Tomato (Lycopersicon esculentum) Plants with Reduced Ethylene Synthesis, Plant Physiol, 102:991-916, (1993).
Picton, Steve, et al., Altered fruit ripening and leaf senescence in tomatoes expressing an antisense ethylene-forming enzyme transgene, The Plant Journal, 3(3):469-481, (1993).
Pua, Eng-Chong, et al., Ethylene Regulating Shoot Regenerability in Vitro, Rice Biotechnology Quarterly, 21:22-23, (1994).
Woodson, William R., et al., Expression of Ethylene Biosynthetic Pathway Transcript in Senescing Carnation Flowers.sup.1, Plant Physiol, 99:0526-0532, (1992).
Nakagawa, Naoki, et al., Cloning of a Complementary DNA for Auxin-Induced 1-Aminocyclopropane-1carboxylate Synthase and Differential Expression of the Gene by Auxin and Wounding, Plant Cell Physiol, 32(8):1153-1163, (1991).
Oeller, Paul W., et al., Reversible Inhibition of Tomato Fruit Senescence of Antisense RNA, Science 254:437-439, (1991).
Olson, David, C., et al., Differential Expression of two genes for 1-aminocyclpropane-1-carboxylate synthase in tomato fruits, Proc. Natl. Acad. Sci. USA, 88:5340-5344, (1991).
Park, Ky Young, et al., Molecular cloning of an 1-aminocyclopropane-1-carboxylate synthase from senescing carnation flower petals, Plant Molecular Biology, 18:377-386, (1992).
Rottmann, William H., et al., 1-Aminocyclopropane-1-Carboxylate Synthase in Tomato is Encoded by a Multigene Family Whose Transcription is Induced During Fruit and Floral Senescence, J. Mol. Biol. 222:937-961, (1991).
Lay-Yee M. et al., Isolation of A Putative Full Length cDNA Coding For Apple ACC Synthase, Plant Physiol, 102(1): Supp., 1993, Abstract 581.
Masayasu, N., et al., Biological Abstract 94(12), Abstract 130960, (1992).
Wagoner, Wendy, J., et al., Superior Regenerat
Boeshore Maury L.
Carney Kim J.
Deng Rosaline Z.
Reynolds John F.
Ruttencutter Glen E.
Nelson Amy J.
Seminis Vegetable Seeds Inc.
Smith Lynette R. F.
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