Transgenic plants expressing a cellulase

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

07838732

ABSTRACT:
The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and/or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

REFERENCES:
patent: 4904599 (1990-02-01), Ozaki
patent: 5168064 (1992-12-01), Bennett et al.
patent: 5393670 (1995-02-01), Knowles
patent: 5457046 (1995-10-01), Wöldike
patent: 5470725 (1995-11-01), Borriss et al.
patent: 5475101 (1995-12-01), Ward
patent: 5543576 (1996-08-01), van Ooijen et al.
patent: 5545818 (1996-08-01), McBride et al.
patent: 5614395 (1997-03-01), Ryals et al.
patent: 5705375 (1998-01-01), van Ooijen et al.
patent: 5925806 (1999-07-01), McBride et al.
patent: 5981835 (1999-11-01), Austin-Phillips et al.
patent: 0332104 (1989-09-01), None
patent: 0449376 (1991-02-01), None
patent: 0479359 (1992-08-01), None
patent: 0589841 (1994-03-01), None
patent: WO90/09436 (1990-08-01), None
patent: WO9116440 (1991-10-01), None
patent: WO9201042 (1992-01-01), None
patent: WO9520668 (1995-03-01), None
patent: WO9514099 (1995-05-01), None
patent: WO9516783 (1995-06-01), None
patent: WO9525787 (1995-09-01), None
patent: WO9604781 (1996-02-01), None
US 5,536,635, 07/1996, Thomas et al. (withdrawn)
B. Henrissat et al., Cellulase families revealed by hydrophobic cluster analysis,Gene, 81(1989):83-95.
N. R. Gilkes et al., Domains in Microbial β-1,4-Glycanases: Sequence Conservation, Function, and Enzyme Families,Microbiological Reviews, Jun. 1991, p. 303-315.
Lashbrook et al., Functional Analysis of Cx-Cellulase (Endo β-1-4-Glucanase) Gene Expression in Transgenic Tomato Fruit, Cellular and Molecular Aspects of the Plant Hormone Ethylene. J.C. Pech et al. (eds.) (Kluwer Academic Publishers), (1993), pp. 123-128.
Collmer, A. et al. Cloning and Expression of a Thermomonospora YX Endocellulase Gene inE. coli Bio/Technology, (Sep. 1983), pp. 594-601.
Ghangas, G.S et al., Cloning of theThermomonospora fuscaEndoglucanase E2 Gene inStreptomyces lividans: Affinity Purification and Functional Domains of the Cloned Gene ProductApplied and Environmental Microbiology, vol. 54, No. 10 (Oct. 1988), pp. 2521-2526.
Jung et al, DNA Sequences and Expression inStreptomyces lividansof an Exoglucanase Gene and an Endoglucanase Gene fromThermomonospora fusca Applied and Environmental Microbiology, vol. 59, No. 9 (Sep. 1993), pp. 3032-3043.
Lao et al, DNA Sequences of Three β-1, 4-Endoglucanase Genes fromThermomonospora fusca Journal of Bacteriology, vol. 173, No. 11 (Jun. 1991), pp. 3397-3407.
Wilson, D.B., Biochemistry and Genetics of Actinomycete CellulasesCritical Reviews in Biotechnology, vol. 12(1/2) (1992), pp. 45-63.
Lashbrook et al. Two Divergent Endo B-1, 4-glucanase Gene Exhibit Overlapping Expression in Ripening Fruit and Abscising Flowers, Oct. 1994, The Plant Cell, vol. 6, pp. 1485-1493.
Yoshikawa et al. Resistance to Fungal Disease in transgenic Tobacco Plants Expressing the . . . , 1993, Naturwissenschaften, vol. 80, pp. 417-420.
Takeuchi et al. Molecular Cloning and Ethylene Induction of mRNA Encoding a Phytoalexin . . . , 1990, Plant Physiol., vol. 93, pp. 673-682.
Melchers et al. Extracellular Targeting of the Vacuolar Tobacco Proteins AP24, Chitinase and B-1, 3-glucanase in Transgenic Plants, 1993, Plant Molecular Biology, vol. 21, pp. 583-593.
Aspegren, et al., Secretion of a heat stable fungal beta-glucanase from transgenic suspension-cultured barley cellsMolecular Breeding, vol. 1 (1995) pp. 91-99.
Hausmann, R., “The T7 Group.” in ed. Calendar, Richard,The Bacteriophages(New York and London, Plenum Press, 1988), pp. 259-289.
Heifetz, et al., Chemical Regulation of nuclear and plastid transgenes in plants,Supplement to Plant Physiology, vol. 114(3) (Jul. 1997) pp. 308.
Kotani, et al., Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymeraseNucleic Acids Research, vol. 15 (1987) pp. 2653-2664.
McBride, et al., Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymeraseProceedings of the National Academy of Science of USA, vol. 91 (Jul. 1994) pp. 7301-7305.
McGraw, et al., Sequence and analysis of the gene for bacteriophage T3 RNA polymeraseNucleic Acids Research, vol. 13 (1985) pp. 6753-6766.
Pen, et al., Production of ActiveBacillus licheniformisAlpha-Amylase in Tobacco and its ApplicationBio/Technology, vol. 10(3) (Mar. 1992) pp. 292-296.
Hebers, K., et al. A thermostable xylanase fromClostridium thermocellumexpressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified,Bio/technologyvol. 13:63-66 (Jan. 1995).
Jung et al., Applied and Environmental Microbiology, “DNA Sequences and Expression inStreptomyces lividansof an Exoglucanase Gene and an Endoglucanase Gene fromThermomonospora fusca”, (1993) 59(9):3032-3043.
Hu et al., Gene, “Cloning ofThermomonospora fuscaGenes Coding for Beta 1-4 Endoglucanases E1, E2, and E5” (1988) 331-337.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Transgenic plants expressing a cellulase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic plants expressing a cellulase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic plants expressing a cellulase will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4193559

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.