Transgenic plants displaying multiple virus resistance and a pro

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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435 691, 4351723, 4352404, A01H 500, C12N 1582

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active

055896251

ABSTRACT:
This invention discloses transgenic plants, such as transgenic tobacco and potato, having resistance to multiple viral taxonomic groups using parts of the 2,5A oligoadenylate pathway. In particular, said plants are genetically engineered to contain a DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity. By this means a step in the 2,5A oligoadenylate pathway heretofore believed to be missing in all plants is provided so that viral infection in the transgenic plants is inhibited via a 2,5A dependent endonuclease. Moreover, this invention relates to a process for the production of said transgenic plants by transfection with a genetically engineered DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity.

REFERENCES:
Truve, et al., Transgenic Potato Palnts Expressing Mammalian 2'5-5'Oligoadenylate Synthetase are Protected FromPotato Virus X Infection Under Field Conditions, Biotechnology, vol. 11, Sep. 1993, pp. 1048-1052.
Truve, et al., Principles and background for the construction of transgenic plants displaying multiple virus resistance, Arch Virol (1994) [Suppl] 9:41-50.
Rutherford, et al., The murine 2-5A synthetase locus: three distinct transcripts form two linkd genes, Nucleic Acids Research, vol. 19, No. 8, pp. 1917-1924, (1991).
Potrykus, Ingo, Gene Transfer to Cerals: An Assessment, BioTechnology, vol. 8, pp. 535-542, Jun. 1990.
Devash, et al., 5'-Dephosphorylated 2',5'-Adenylate Trimer and Its Analogs, The Journal of Biological Chemistry, vol. 259, No. 6, Issue of March 25, pp. 3482-3486, 1984.
Babosha, et al., Biological Abstracts, Ref. No. 66022, vol. 91 (1991).
Schroder, et al., Protection of HeLa-T4.sup.+ cells against human immunodeficiancy virus (HIV) infection after stable transfection with HIV LTR-2.degree.,5'-Oligoadenylate synthetase hybrid gene.sup.1, Research Communications, vol. 4, pp. 3124-3130, Oct. 1990.
Sela, et al., Resistance systems related to the N gene and their comparison with interferon, 1987 Plant Resistance to Viruses., Wiley, Chichester (Ciba Foundation Symposium 133) P 109-119.
Gelvin, Stanton, Accurate expression of genes in transgenic plants: do fundamental differences exist between eukaryotes?, Plant Molecular Biology, 8:355-359, (1987).

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