Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2004-06-07
2009-06-09
Kallis, Russell (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S312000, C800S314000, C800S317000, C800S320100, C536S023100, C536S023200, C536S023600, C435S320100, C435S419000
Reexamination Certificate
active
07544863
ABSTRACT:
Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
REFERENCES:
patent: 5306862 (1994-04-01), Chappell et al.
patent: 5349126 (1994-09-01), Chappell et al.
patent: 5365017 (1994-11-01), Chappell et al.
patent: 5460949 (1995-10-01), Saunders et al.
patent: 5480805 (1996-01-01), Wolf et al.
patent: 5589619 (1996-12-01), Chappell et al.
patent: 6153815 (2000-11-01), Covello
patent: 0480730 (1992-04-01), None
patent: 09121863 (1997-05-01), None
patent: WO 93/02187 (1993-02-01), None
patent: WO 97/03202 (1997-01-01), None
patent: WO 97/34003 (1997-09-01), None
patent: WO 98/45457 (1998-10-01), None
patent: WO 99/04622 (1999-02-01), None
patent: WO 00/61771 (2000-10-01), None
patent: WO 01/31027 (2001-03-01), None
Chappell J. et al. Plant Physiology; 1995, vol. 109, pp. 1337-1343.
Schaeffer A. et al. Lipids, 2000; vol. 35, No. 3, pp. 263-269.
Bach and Benveniste, “Cloning of cDNAs or genes encoding enzymes of sterol biosynthesis from plants and other eukaryotes: heterologous expression and complementation analysis of mutations for functional characterization,”Progress in Lipid Research, 36(2/3): 197-226, 1997.
Bak et al., “Cloning and expression inEscherichia coliof the obtusifoliol 14-alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14-alpha-demethylases (CYP51) from fungi and mammals,”Plant Journal, 11(2):191-201, 1997.
Bak et al., “Cloning and expression inEscherichia coliof the obtusifoliol 14-alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14-alpha-demethylases (CYP51) from fungi and mammals,”EMBL Online!, Database Accession No. U74319, abstract, 1996.
Bard et al., “Genetic and biochemical aspects of yeast sterol regulation involving 3-hydroxy-3-methylglutaryl coenzyme A reductase,”J. General Microbiology, 125:415-420, 1981.
Basson et al., “Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis,” Molecular Cellular Biology, 8(9):3797-3808, 1998.
Broun et al., “Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids,”Science, 282:1315-1317, 1998.
Cabello-Hurtado et al., “Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14-alpha-demethylase (CYP51) in wheat,”Biochemical and Biophysical Research Communications, 230(2):381-385, 1997.
Cabello-Hurtado et al., “Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14-alpha-demethylase (CYP51) in wheat,”EMBL Online!, Database Accession No. Y09291, abstract, 1996.
Chin et al., “Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endloplasmic reticulum,”Nature, 308(5960):613-617, 1984.
Colebatch et al., “Lotus faponicus root nodule ESTs: tools for functional genomics,”EMBL Online!, Database Accession No. AW719774, abstract, 2000.
Covello, “An example of intron junctional sliding in the gene families encoding squalene monoxygenase homologues in Arabidopsis thaliana and Brassica napus,”EMBL Online!, Database Accession No. AJ005930, 1998.
Dale et al., “Bacterial expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-CoA reductase (isoform HMGR1) from arabidopsis thaliana, and its inactivation by phosphorylation at Ser577 by brassica oleracea 3-hydroxy-3-methylglutaryl-CoA reductase kinase,”Eur. J. Biochem.; 233:506-513, 1995.
Deavarenne, “Regulation of squalene synthase, a key enzyme of sterol biosynthesis, in tobacco,”Plant Physiology, 129:1095-1106, 2002.
Downing et al., “The isolation of two mutants of saccharomyces cerevisiae which demonstrate increased activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase,”Biochemical and Biophysical Research Communications, 94(3):974-979, 1980.
Fourgoux-Nicol et al., “Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte,”Plant Molecular Biology, 40:857-872, 1999.
Gill et al., “Membrane-bound domain of HMG CoA reductase is required for sterol-enhanced degradation of the enzyme,”Cell, 41:249-258, 1985.
Gonzalez et al., Abstract of poster at Third Terpnet Meeting of the European Network on Plant Isoprenoids, May 29-30, Poiters, France, 1997.
Jenkins et al., “Plant sterols, health claims and strategies to reduce cardiovascular and strategies to reduce cardiovascular disease risk,”J. of the American College of Nutrition, 18:559-562, 1999.
Nakamura et al., “A large scale analysis if cDNA in Aradopsis thalian: generation of 12,028 non-redundant expressed sequence tags from normalized size-selected cDNA libraries,”EMBL Online!, Database Accession No. AV440215, 2000.
Nguyen, “The cholesterol-lowering action of plant stanol esters,”J. of Nutrition, 129:2109-2112, 1999.
Register et al., “Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment,”Plant Mol. Biol., 25:951-961, 1994.
Schafer et al., “An example of intron junctional sliding in the gene families encoding sqalene monooxygenase homologues in Arabidopsis thaliana and Brassica napus,”Plant Molecular Biology, 39(4):721-728, 1999.
Schaller et al., “Overexpression of an arabidopsis cDNA encoding a sterol-C24(l)-methyltransferase in tobacco modifies the ration of 24-methyl cholesterol to sitosterol and is associated with growth reduction,”Plant Physiol., 118:461-469, 1998.
Schaller et al., “Expression of the hevea brasiliensis (H.B.K.) müll. Arg. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 in tobacco results in sterol overproduction,”Plant Physiol., 109:761-770, 1995.
Shintani et al., “Elevating the vitamin E content of plants through metabolic engineering,”Science, 282(5396):2098-2100, 1998.
Tada and Shiroishi, “Mechanism of photoregulated cartogenesis in Rhodoturul minuta v. photoinduction of 3-hydroxy-3-methyl glutaryl coenzyme A reductase,”Plant and Cell Physiol., 23(4):615-621, 1982.
Van der Hoeven et al., “Generation of ESTs retrieved from tomato radicule tissue,”EMBL Online!, Database Accession No. AW625933, abstract, 2000.
Yoder et al., “Transformation systems for generating marker-free transgenic plants,”Bio/Technology, 12:263-267, 1994.
Karunanandaa Balasulojini
Kishore Ganesh M.
LeDeaux John R.
Post-Beittenmiller Martha
Thorne Gregory M.
Kallis Russell
Li Esq. Chunping
Monsanto Technology LLC
Sonnenschein Nath & Rosenthal LLP
LandOfFree
Transgenic plants containing altered levels of steroid... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Transgenic plants containing altered levels of steroid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic plants containing altered levels of steroid... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4074114