Transgenic plants and method for node segment transformation

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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435469, 435468, 435410, 435419, 435421, 435430, 4352523, 800278, 800293, 800298, 800320, C12N 1582, C12N 504, A01H 400, A01H 510

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059489561

ABSTRACT:
Transgenic monocotyledonous plants are produced by inserting a foreign genetic material directly into a node segment of a stem of a plant and thereafter subjecting the node segment to conditions sufficient to permit regeneration of the node segment into a plantlet. Preferably, the genetic material is inserted by biolistic transformation, Agrobacterium, or direct DNA uptake mediated by electroporation. The process is especially useful for producing transgenic ryegrasses, fescues and turfgrasses, such as St. Augustinegrass, creeping bentgrass, Kentucky bluegrass, and the like.

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