Transgenic pigs

Details Transgenic pigs Transgenic pigs

1994-04-12

1996-11-12

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 435 694, 800 2, 800DIG1, 935 13, C12N 1500

Patent

active

055739334

ABSTRACT:
A method for preparing transgenic pigs is disclosed. The method comprises the steps of: obtaining a recently fertilized pig ovum; isolating a first DNA sequence encoding a suitable promoter region; inserting the first DNA sequence into a plasmid cloning vector; isolating a second DNA sequence encoding a porcine growth hormone gene; inserting the second DNA sequence into the plasmid cloning vector; introducing the plasmid cloning vector into the male pronucleus; and, subsequently implanting the ovum into a female pig.

REFERENCES:
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J. Brosius et al., "Spacing of the --10 and --35 Regions in the Tac Promoter", J. Biol. Chem., 260(6):3539-3541 (Mar. 1985).
H. A. de Boer et al., "The tac promoter: A functional hybrid derived from the trp and lac promoters", PNAS-USA, 80:21-25 (Jan. 1983).
F. M. DeNoto et al., "Human Growth Hormone DNA Sequence and mRNA Sequence: Possible Alternative Splicing", Nucl. Acids Res., 9:3719-3730 (1981).
Hsiung et al. (Becker and Hsiung), "Expression, Secretion and Folding of Human Growth Hormone in Escherichia coli", Febs. Letters, 204(1):149-150 (1986).
Robins, personal communication, (1990).
Kadonaga et al., "Promoter-specific Activation of RNA Polymerase II Transcription of Spl", Trends in Biochem. Sci., 11:20-23 (1986).
Nonander et al., "Construction of Improved M13 Vectors Using Oligodeoxynucleotide-Directed Mutagenesis", Gene, 26:101-106 (1981).
Seeburg et al., "Efficient Bacterial Expression of Bovine and Porcine Growth Hormones", DNA 2:37-45 (1983).
Tranplantation of the Human Insulin Gene into Fertilized Mouse Eggs.
Metallothionein-Human GH Fusion Genes Stimulate Growth of Mice.
Dramatic Growth of Mice that Develop from Eggs Microinjected with Metallothionein-Growth Hormone Fusion Genes.
Developmental Fate of a Human Insulin Gene in Transgenic Mouse.
Production of Transgenic Rabbits, Sheep and Pigs by Microinjection.
Isolation and Characterization of the Porcine Growth Hormone Gene.
Development of 1-Cell and 2-Cell Pig Ova After Microinjection of Genes.
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