Transgenic non-human mammals producing oligosaccharides and glyc

Details Transgenic non-human mammals producing oligosaccharides and glyc Transgenic non-human mammals producing oligosaccharides and glyc

1996-09-10

1999-04-06

Crouch, Deborah

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

800DIG1, 435 691, 4351723, 935 60, C12N 500, C12N 1500, C12P 2106

Patent

active

058920706

ABSTRACT:
The invention relates to transgenic non-human mammals characterized in that the genome of said mammals contain at least one heterologous gene encoding for the production of heterologous catalytic entity selected from the group consisting of enzymes and antibodies, and wherein said catalytic entity produces a second heterologous product in the milk of said mammal. Especially useful in the practice of the invention are human glycosyltransferases and transgenic sheep, goats and cows. The heterologous product includes oligosaccharides and glycoconjugates. Specifically exemplified, is the production of 2'-fucosyl-lactose in the milk of transgenic mice which contain and express a transgene encoding .alpha.-1,2-fucosyltransferase operatively linked to a mammary gland specific promoter.

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