Transgenic mouse expressing human FcRn protein

Details Transgenic mouse expressing human FcRn protein Transgenic mouse expressing human FcRn protein

2008-04-15

2008-04-15

Li, Q. Janice (Department: 1633)

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

Transgenic nonhuman animal

C800S008000, C800S018000, C800S021000, C800S022000, C800S024000, C800S025000

Reexamination Certificate

active

11235996

ABSTRACT:
Disclosed is a transgenic knockout mouse whose genome comprises a homozygous disruption in its endogenous FcRn gene. The homozygous RcRn disruption prevents the expression of a functional FcRn protein, resulting in a transgenic knockout mouse in which exogenously administered IgG1 exhibits a substantially shorter half-life, as compared to the half-life of exogenously administered IgG1 in a wild-type mouse. The transgenic knockout mouse with a homozygous RcRn disruption is also unable to absorb maternal IgG in the prenatal or neonatal stage of development. Also disclosed is a transgenic knockout mouse comprising a homozygous FcRn disruption and a human FcRn transgene. The transgenic addition of human FcRn results in a substantial increase in the half-life of exogenously administered human IgG1. Methods of using the transgenic knockout mouse, and cells derived from them, are also disclosed.

REFERENCES:
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5225539 (1993-07-01), Winter
patent: 5530101 (1996-06-01), Queen et al.
patent: 5585089 (1996-12-01), Queen et al.
patent: 5693761 (1997-12-01), Queen et al.
patent: 5877396 (1999-03-01), Ravetch et al.
patent: 6096871 (2000-08-01), Presta et al.
patent: 6121022 (2000-09-01), Presta et al.
patent: WO-2005/013912 (2005-02-01), None
Pearson, Nature 2002;415:8-9.
Linder, Lab Animal May 2001;30:34-9.
Nebert et al, Biochemical Pharmacol Feb. 1997;53:249-54.
Mullins et al, J Clin Invest Apr. 1996;97:1557-60.
Logan and Sharma, Clin Exp Pharmacol Physiol Dec. 1999;26:1020-25.
Lupus erythematosus. Wikipedia 2007.
Theofilopolos and Dixon,Adv. Immunol. 37:269-390 (1985).
Raghavan et al.,Immunity 1:303-315 (1994).
Morel et al.,Immunity 1:219-229 (1994).
Murphy and Roths,Arthritis Rheum. 22:1188-1194 (1979).
Andrews et al.,J. Exp. Med. 148:1198-1215 (1978).
Simister and Mostov,Nature 337:184-7 (1989).
Simister et al.,Eur. J. Immunol. 26:1527-31 (1996).
Ghetie et al.,Eur. J. Immunol. 26:690-696 (1996).
Ghetie and Ward,Annu. Rev. Immunol. 18:739-66 (2000).
Brambell et al.,Nature 203:1352-5 (1964).
Brambell, F.W.,Lancet. 2:1087-93 (1966).
Capecchi, M.R.,Trends Genet. 5:70-76 (1989).
Israel et al.,Immunology 89:573-578 (1996).
Karlsson et al.,Proc. Natl. Acad. Sci. USA 96:2244-2249 (1999).
Israel et al.,J. of Immun. 154:6246-6251 (1995).
Zhiya Yu et al., “Mechanism of Intravenous Immune Globulin Therapy in Antibody-Mediated Autoimmune Diseases,” New England Journal of Medicine 340(3):227-228 (1999).
Amos Etzioni et al., “High Dose Intravenous Gammaglobulins in Autoimmune Disorders: Mode of Action and Therapeutic Uses,” Autoimmunity 3:307-315 (1989).
Robert A. Good et al., “Historic Aspects of Intravenous Immunoglobulin Therapy,” Cancer 68:1416-1419 (1991).
Akilesh et al., “The MHC class I-like Fc receptor promotes humorally mediated autoimmune disease,”The Journal of Clinical Investigation, 113(9):1328-1333 (2004).
Chaudhury et al., “The major histocompatibility complex-related Fc receptor for lgG (FcRn) binds albumin and prolongs its lifespan,”Journal of Experimental Medicine, 197(3):315-322 (2003).
Getman et al., “Pharmacokinetic effects of 4C9, and anti-FcRn antibody, in rats: implications for the use of FcRn inhibitors for the treatment fo humoral autoimmune and alloimmune conditions,”Journal of Pharmaceutical Sciences, 94(4): 718-729 (2005).
Medesan et al., “Comparative Studies of Rat IGG to Further Delineate the FC: FCRN Interaction Site,”European Journal of Immunology, 28(7):2092-2100 (1998).
Ghetie et al., “Increasing the serum persistence of an IgG fragment by random mutagenesis,” Nature Biotechnology 15:637-640 (1997).

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