Transgenic mice knockouts of BACE-1

Details Transgenic mice knockouts of BACE-1 Transgenic mice knockouts of BACE-1

2007-12-18

2007-12-18

Crouch, Deborah (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

Transgenic nonhuman animal

C800S003000, C800S008000, C800S012000, C800S025000, C435S354000

Reexamination Certificate

active

10082804

ABSTRACT:
The invention provides transgenic animals that are heterozygous or homozygous for a nonfunctional allele of BACE-1. These animals show normal development indicating that BACE-1 is not necessary for development and hence is an appropriate therapeutic target. The transgenic animals are useful in various screening assays including identifying inhibitors of other enzymes involved in processing of APP to Aβ and determining a toxicity profile of inhibitors of BACE-1.

REFERENCES:
patent: 4666829 (1987-05-01), Glenner et al.
patent: 5612486 (1997-03-01), McConlogue et al.
patent: 5811231 (1998-09-01), Farr et al.
patent: 6040138 (2000-03-01), Lockhart et al.
patent: 6114133 (2000-09-01), Seubert et al.
patent: 6204061 (2001-03-01), Capecchi et al.
patent: 2002/0157122 (2002-10-01), Wong et al.
patent: WO 97/07669 (1997-03-01), None
patent: WO97/07669 (1997-03-01), None
patent: WO 98/30683 (1998-07-01), None
patent: WO98/30683 (1998-07-01), None
patent: WO98/37183 (1998-08-01), None
patent: WO 98/37183 (1998-08-01), None
patent: WO98/39416 (1998-09-01), None
patent: WO 98/39416 (1998-09-01), None
patent: WO99/37143 (1999-07-01), None
patent: WO 99/37143 (1999-07-01), None
patent: WO 00/17369 (2000-03-01), None
patent: WO 00/47618 (2000-08-01), None
patent: WO 00/72880 (2000-12-01), None
Simerly, C. et al. Molecular Correlates of Primate Nuclear Transfer Failures. Science. Apr. 11, 2003, vol. 300, p. 297.
Mitalipov et al. Rheusus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells. Biology of Reproduction. 2002, vol. 66, pp. 1367-1373.
Polejaeva et al. Cloned Pigs Produced by Nuclear Transfer from Adult Somatic Cells. Nature. Sep. 7, 2000, vol. 407, pp. 86-90.
Pennisi et al. Clones: A Hard Act to Follow. Science. Jun. 9, 2000, vol. 288, pp. 1722-1727.
Westhusin et al. Clonin gto Reproduce Desired Genotypes. Theriogenology. 2001, vol. 55, pp. 35-49.
Meirelles et al. Complete Replacement of the Mitochondrial Genotype in a Bos indicus Calf Reconstructed by Nuclear Transfer to a Bos taurus Oocyte. Genetics 2001, vol. 158, pp. 351-356.
Fehilly et al. Cytogenetic and Blood Group Studies of Sheep/Goat Chimaeras. J. Reproduct. Fertility. 1985, vol. 74, pp. 215-221.
Denning, C. et al. Gene Targeting in Primary Fetal Fibroblasts from Sheep and Pig. Cloning and Stem Cells. 2001, vol. 3, O. 4, pp. 221-231.
Wheeler, M. B. et al. Transgenic Technology and Applications in Swine. Theriogenelogy. 2001, vol. 56, pp. 1345-1369.
Moreadith, R. W. Gene Targeting in Embryonic Stem Cells: the New Physiology and Metabolism. J. Molecular Med. 1997, vol. 75, pp. 208-216.
Prelle, K. et al. Establishment of Pluripotent Cell Lines from Vertebrate Species—Present Status and Future Prospects. Cells Tissues Organs. 1999, vol. 165, pp. 220-236.
Gardner, R. L. et al. Reflections on the Biology of Embryonic Stem Cells. Internat. J. Devel. 1997, vol. 41, pp. 235-243.
Games D. et al. Alzheimer-Type Neuropathology in Transgenic Mice Overexpressing V717F Beta-Amlyloid Precursor Protein. Nature. Feb. 9, 1995, vol. 373, pp. 523-527.
Mansour, S. L. et al. Disruption of the Proto-Oncogene Int-2 in Mouse Embryo-Derived Stem Cells: A General Strategy for Targeting Mutations to Non-Selectable Genes. Nature. Nov. 24, 1988 vol. 336, pp. 348-352.
Farhangrazi, Z. S. et al. High Density Lipoprotein Decreases Beta-Amyloid Toxicity in Cortical Cell Culture. NeuroReport. Mar. 24, 1997, vol. 8, No. 5, pp. 1127-1130.
Cllark, A. J. et al. Gene Targeting in Livestock: A Preview. Transgenic Research. 2000, vol. 9, pp. 263-275.
Farzan et al. BACE2, a Beta Seretase Homolog, Cleaves a the Beta Site and within the Amyloid-Beta Region of the Amyolid-Beta Precursor Protein. Proceedings National Acad. Sci. USA. Aug. 15, 2000, vol. 97, No. 17, pp. 9712-9717.
Hardy, J. Amyloid, the Presenilins and Alzheimer's Disease. Trends in Neuroscience. 1997, vol. 20, No. 4, pp. 154-159.
U.S. Appl. No. 60/271,092, filed Feb. 23, 2001.
U.S. Appl. No. 60/271,514, filed Feb. 26, 2001.
U.S. Appl. No. 60/293,762, filed May 25, 2001.
Acquati et al., “The gene encoding DRAP (BACE2), a glycosylated transmembrance protein of the aspartic protease family, maps to the Down critical region,”FEBS Letters468(1):59-64 (2000).
Altschul et al., “Basic Local Alignment Search Tool,”J. Mol. Biol. 215:403-410 (1990).
Borchelt et al., “Accelerated Amyloid Deposition in the Brains of Transgenic Mice Coexpressing Mutant Presenilin 1 and Amyloid Precursor Proteins,”Neuron19:939-945 (1997).
Bradley et al., “Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines.”Nature309:255-256 (1984).
De Strooper et al., “Deficiency of presenilin-1 inhibits the normal cleavage of amyloid precursor protein,”Nature391:387-390 (1998).
Farzan et al., “BACE2, a β-secretase homolog, cleaves at the β site and within the amyloid-β region of the amyloid-β precursor protein,”Proc. Nat'l. Acad. Sci. 97(17):9712-9717 (2000).
Glenner & Wong, “Alzheimer's Disease and Down's Syndrome: Sharing of a Unique Cerebrovascular Amyloid Fibril Portein,”Biochemical and Biophysical Research Communications120:1131 (1984).
Hardy et al., “Amyloid, the presenilins and Alzheimer's disease,”TINS20:154-159 (1997).
Henikoff & Henikoff, “Amino acid substitution matrices from protein blocks,”Proc. Nat'l Acad. Sci. USA89:10915-10919 (1992).
Hsiao et al., “Correlative Memory Deficits, Aβ Elevation, and Amyloid Plaques in Transgenic Mice,”Science274:99-102 (1996).
Huber et al., “cDNA Cloning and Molecular Characterization of Human Brain Metalloprptease MP100: A β-Secretase Canadidate?”Journal of Neurochemistry72:1215-1223 (1999).
Jaenisch et al., “Transgenic animals,”Science240:1468-1474 (1988).
Johnson-Wood et al., “Amyloid precursor protein processing and Aβ42deposition in a transgenic mouse model of Alzheimer disease,”Proc. Nat'l Acad. Sci. USA94:1550-1555 (1997).
Kang et al., “The Precursor of Alzheimer's Disease Amyloid A4 Protein Resembles a Cell-Surface Receptor,”Nature325:733-736 (1987).
Karlin & Altschul, “Applications and statistics for multiple high-scoring segments in molecular sequences,”Proc. Nat'l. Acad. Sci. USA90:5873-5787 (1993).
Kitaguchi et al., “Novel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activity,”Nature331:530 (1988).
Lai et al., “Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning,”Science295:1089-1092 (2002).
Needleman & Wunsch., “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins,”J. Mol. Biol. 48:443 (1970).
Pearson & Lipman, “Improved tools for biological sequence comparison,”Proc. Nat'l Acad. Sci. USA85:2444-2448 (1988).
Ponte et al., “A New A4 Amyloid mRNA contains a domain homologous to serine proteinase inhibitors,”Nature331:525-527 (1988).
Roberds et al., “BACE knockout mice are healthy despite lacking the primary β-secretase activity in brain: implications for Alzheimer's disease therapeutics”Human Molecular Genetics10:1317-1324 (2001).
Roger et al., “Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment,”Mamm. Genome8:711-713 (1997).
Selkoe et al., “Normal and Abnornal Biology of the β-Amyloid Precursor Protein,”Annual Rev. Neurosci17:489-517 (1994).
Sinha et al., “Cellular mechanisms of β-amyloid production and secretion,”Proc. Natl. Acad. Sci. USA96:11049-11053 (1999).
Sinha et al., “Purification and cloning of amyloid precursor protein β-secretase from human brain,”Nature402:537-540 (1999).
Smith & Waterman, “Comparison of Biosequences,”Adv. Appl. Math. 2:482 (1981).
Sturchler-Pierrat et al.

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