Transgenic mice expressing human insulin

Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal

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800 3, 800 14, 800 25, A01K 6700, A01K 67033, C12N 1500, G01N 3300

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060180978

ABSTRACT:
A process for producing a transgenic mouse which contains and expresses the human insulin hormone gene. The insulin gene is expressed only in the pancreas of the transgenic mouse and is regulated by glucose, glucagon, or other insulin affectors in a manner which is indistinguishable from that of normal mice. Progeny of the trangenic mice inherit the human insulin gene in a Mendelian manner, with approximately 50% of the mice of each litter expressing the human insulin gene. The weights of the transgenic mice, growth rate, feeding behavior, reproductive capability, and longevity appear indistinguishable from normal mice. The mice are useful for studies of pharmacokinetics of insulin expression and for investigations of possible drug interactions with glucose homeostasis.

REFERENCES:
patent: 4431740 (1984-02-01), Bell et al.
patent: 4564517 (1986-01-01), Fudenberg et al.
patent: 4736866 (1988-04-01), Leder et al.
patent: 4870009 (1989-09-01), Evans et al.
patent: 4873191 (1989-10-01), Wagner et al.
Palmiter et al Cell 41, 343-345 (1985).
Stewart, T.A., et al., "Human .beta.-Globin Gene Sequences Injected into Mouse Eggs, Retained in Adults, and Transmitted to Progeny, " Science 217:1046-1048 (1982).
Constantini, F., and Lacy, E., "Introduction of rabbitt .beta.-globin Gene into the Mouse Germ Line," Nature294:92-94 (1981).
Katsuki, M., et al., "Experimental System with Transgenic Mouse" Protein, Nucleic Acid , Enzymes 40:2001-2007 (1995).
Palmiter, et al., "Differential Regulation of Metallothionein Thymidine Kinase Fusion Genes in Transgeric Mice and Their Offspring," Cell29:701-710 (1982).
Bucchini et al., Proc. Natl. Acad. Sci. USA 83: 2511-2515 (Apr. 1986).
Jami et al. Journal of In Vitro Fertilization and Embryo Transfer (1986), Biological Abstracts Reference No. 31074293.
Jami et al., J. In. Vitro Fert. Embryo Transfer 3:70 (1986).
Selden et al., Nature 321:525-528 (1986).
Brinster, Ralph L and Palmiter, Richard D., "Introduction of Genes into the Germ Line of Animals," Friends of the Harvey Society , 1-38 (1984-1985).
Brinster, Ralph L and Palmiter, Richard D., "Germ-Line Transformation of Mice," Ann. Rev. Genet. 20:465-99 (1986).
McDonald, Raymond J. et al. "Transgenic Progeny Inherit Tissue-Specific Expression of Rat Elastase I Genes," DNA vol. 5 5:393-401 (1986).
Hammer, Robert E. et al., "Estrogen Regulation of the Avian Transferrin Gene in Transgenic Mice," Molecular and Cellular Biology , vol. 6: (4)1010-1014 (1986).
Hammer, Robert E. et al., "Genetic Engineering of Mammalian Embryos.sup.1,2,3," J. Anim. Sci. 63:269-278 (1986).
Pursel, V.G. et al., "Progress on Gene Transfer in Farm Animals," Veterinary Immunology and Immunopathology, 17:303-312 (1987).
Hammer, Robert E. et al., "Genetic Engineering of Livestock," vol. 244:1281-2257 (1989).
Allison, Janette et al., "Tissue-Specific and Hormonal Regulation of the Gene for Rat Prostatic Steroid-Binding Protein in Transgenic Mice," Molecular and Cellular Biology, vol. 9(5) :2254-2257 (1989).
Murphy, Carol et al., Regulation of the Human C-reactive Gene in Transgenic Mice, The Journal of Biological Chemistry, Vol. 270 (4) :704-708 (1995).
Corcoran, Colm M. et al., "High-level Regulated Expression of the Human G6PD Gene in Transgenic Mice," Gene, 173:241-246 (1996).
Selden et al., "Glucocorticoid regulation of human hormone expression in transgenic mice", J. of Cell. Bio. vol. Suppl. No. 9B, P. 204 (1985) abstract 0943.
Bucchini et al., "Pancreatic expression of human insulin gene in transgenic mice", Proc. Natl. Acad. Sci. USA, 83:2511-2515 (1986).
Palmiter et al., Cell 41 : 343-345 (1985).
Townes et al., The EMBO Journal 4:1715-1723 (1985).
Stewart et al., Cell 38:627-637 (1984).
Gordon et al, Methods in Enzymology vol. 101 pp. 411-433.
Cappecchi, Cell, 22:479-488 (1980).
Gordon, et al., Proc. Natl. Acad. Scie. USA, 77:7380-7384 (1980).
Wagner, et al., Proc. Natl. Acad. Sci. USA, 78:6376-6380 (1981).
Grosschedl, et al., Cell, 38:647-658 (1984).
Brinster, et al., Nature, 306:332-336 (1983).
Palmiter, et al., Nature, 300:611-615 (1982).
Brinster, et al., Cell, 37:367-379 (1984).
Swift, et al., Cell, 38:639-646 (1984).
Lacy, et al., Cell, 34:343-358 (1983).
Hammer, et al., Nature, 311:65-67 (1984).
McKnight, et al., Cell, 34:335-341 (1983).
Palmiter, et al., Cell, 36:869-877 (1984).
Illmensee, et al., Developemental Biology Using Purified Genes, pp. 607-619 (1981).
Burki, et al., The EMBO Journal, 1:127-131 (1982).
Van Der Putten, et al., Mol Gen Genet, 198: 128-138 (1984).
Bell, et al. Nature, 284;26-32 (1980).
Hoppe, et al., Biology of Reproduction, 8:420-426 (1973).
Bucchini, et al., Proc. Natl. Acad. Sci. USA, 83:2511-2515 (1986).

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