Transgenic mammals expressing mutant GP IIIa

Details Transgenic mammals expressing mutant GP IIIa Transgenic mammals expressing mutant GP IIIa

2006-02-07

2006-02-07

Chen, Shin-Lin (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of using a transgenic nonhuman animal in an in vivo...

C800S018000, C800S021000, C800S022000, C800S025000, C435S320100, C435S455000, C424S093210, C536S023500

Reexamination Certificate

active

06995298

ABSTRACT:
The present invention relates to mammals into which foreign DNA has been introduced or in which various modifications or substitutions have been made to an integrin β subunit, thereby generating transgenic or genetically-engineered non-human mammals. In particular, the present invention provides a transgenic mammal in which the endogenous GP IIIa gene has been replaced with an altered or mutant GP IIIa gene in which one or all of the phosphorylable cytoplasmic tyrosine residues have been replaced with non-tyrosine residues such as phenylalanine. Since the platelets in the blood of the resultant transgenic mammals expressing in wild-type mammals, these genetically-engineered animals provide a critical tool for assessing the importance of the phosphorylation reaction for platelet function. The invention is also useful for studying the effect of the mutant GP IIIa integrin subunit on biological processes other than platelet formation.

REFERENCES:
patent: WO 95/25173 (1995-09-01), None
patent: WO 97/08316 (1997-03-01), None
Wall; Transgenic Livestock: Progress and Prospective for the Future, 1996, Theriogenology 45: 57-68.
Houdebine; Production of pharmaceutical proteins from transgenic animals, 1994, Journal of Biotechnology 34: 269-287.
Hammer et. al.; Genetic Egineering of Mammalian Enbryos. 1996. J. Anim. Sci. 63: 269-278.
Ebert et. al.; A Moloney MLV-Rat Somatitropin Fusion Gene Produces Biologically Active Somatotropin in a Transgenic Pig. 1988, Molecular Endocrinol 2: 277-283.ogy.
Mullins et. al.; Perspectives Series: Molecular Medicine in Genetically Engineered Animals. 1996. J. Clin. Invest. vol. 97, No. 7 1557-1560.
Kappel et. al.; Regulating gene expression in transgenic animals, 1992, Current Opinion in Biotechnology #: 548-553.
Strojek et. al.; The Use of Transgenic Animal Technques for Livestock Improvement ; 1988, Genetic Engineering: Principles and methods vol. 10: 221-246.
Moreadith et. al.; Gene targeting in embryonic stem cells: the new physiology and metabolism, 1997. J. Moi. Med. 75: 208-216.
Kappel et al., 1992, Current Opinion in Biotechnology, vol. 3, p. 548-553.
Sigmund, Jun. 2000, Arterioscler. Thromb. Vasc. Biol., p. 1425-1429.
Houdebine, L-M., 2002, Journal of Biotechnology, vol. 98, p. 145-160.
Baudoin et al. (Apr. 1998) Knockout and knockin of the β1 exon D define distinct roles for integrin splice variants in heart function and embryonic development, Genes Dev. 12:1202-1216. (Cold Spring Harbor Press, NY, USA).
Blystone et al. (Nov. 1997) Requirement of integrin β3tyrosine 747 for β3tyrosine phosphorylation and regulation of αvβ3avidity, J. Biol. Chem. 272:28757-28761 (American Society for Biochemistry and Molecular Biology, Bethesda, MD, USA).
Hanks et al. (1995) Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2, Science 269: 679-682 (American Association for the Advancement of Science, Washington, DC, USA).
Liu et al. (1996) Identification of a functionally important sequence in the cytoplasmic tail of integrin β3by using cell-permeable peptide analogs, Proc. Natl. Acad. Sci. USA 93:11819-11824 (Nat'l. Acad. Sciences, Washington, DC, USA).
International Search Report PCT/US99/08285, mailed Aug. 30, 1999.

Affiliated with

Also associated with

Rating

Transgenic mammals expressing mutant GP IIIa does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic mammals expressing mutant GP IIIa, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic mammals expressing mutant GP IIIa will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3646306