Transgenic mammals expressing human coagulation factor VIII

Details Transgenic mammals expressing human coagulation factor VIII Transgenic mammals expressing human coagulation factor VIII

1994-09-21

1999-03-09

Chambers, Jasemine C.

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

800 15, 800 16, 800 17, 800 18, 800 4, 800 7, 800 21, 435455, C12N 1500, C12N 500

Patent

active

058803270

ABSTRACT:
A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human Factor VIII protein and a signal peptide, where the cis-acting regulatory units are active in mammary gland cells and the signal peptide is active in directing newly expressed Factor VIII into the milk of the animal. The promoter may be a milk protein promoter such as for whey acidic protein, casein, lactalbumin, or beta-lactoglobulin promoter. The transgenic mammals are preferably farm animals, for example, cows, goats, sheep, rabbits and pigs. Concurrent expression of a gene for human von Willebrand's Factor into milk may be used to stabilize newly-secreted Factor VIII.

REFERENCES:
patent: 4757006 (1988-07-01), Toole, Jr. et al.
patent: 4873316 (1989-10-01), Meade et al.
patent: 5288846 (1994-02-01), Quertermous et al.
patent: 5366894 (1994-11-01), Clark et al.
R. Halter et al.; Strategies to express Factor VIII Gene Constructs in the Ovine Mammary Gland, Jan. 1993, Theriogenology 39: 137-149.
Wall R. J.; Transgenic Livestock: Progress and Prospects For The Future, Theriogenology 45: 57-68, 1996.
Kaufman "Genetic Engineering of Factor VIII", Nature, 342:207-8, Nov. 1989.
Paleyenda et al. "The Expression of Therapeutic Proteins in Transgenic Animals", Recombinant Technology In Hemostasis and Thrombosis, 197-209, 1991.
Kaufman "Expression and Structure-Function Properties of Recombinant Factor VIII", Transfusion Medicine Reviews, 6;4:235-46, Oct. 1992.
Mikkelsen et al. "Heterogeneity in the Tyrosine Sulfation of Chinese Hamster Ovary Cell Produced Recombinant FVIII", Biochemistry, 30:6;1533-37, 1991.
Hironaka et al. "Comparative Study of the Sugar Chains of Factor VIII Purified from Human Plasma and from the Culture . . .", The Journal of Biological Chemistry, 267:1-9, 1992.
Foster et al. "Factor VIII Structure and Function", Blood Reviews, 3: 180-91, 1989.
Wood et al. "Expression of Active Human Factor VIII from Recombinant DNA Clones", Nature, 312:330-37, Nov. 1984.
Bondioli et al. Production of Trensgenic Cattle by Pronuclear Injection. Production of Transgenic Cattle, Chapter 22.
Roschlau et al. Gene transfer experiments in cattle. J. Reprod. Fert. Suppl. 38, pp. 153-160. 1989.
Krimpenfort et al. Generation of transgenic dairy cattle using in vitro embryo production. Bio/Technology, vol. 9, pp. 844-847. Sep. 1991.

Affiliated with

Also associated with

Rating

Transgenic mammals expressing human coagulation factor VIII does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic mammals expressing human coagulation factor VIII, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic mammals expressing human coagulation factor VIII will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1322834