Transgenic male sterile plants for the production of hybrid seed

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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435 691, 435 701, 4351723, 536 236, 536 2372, 536 241, 536 245, 800250, 47 58, 47DIG1, A01H 500, C12N 1582, C12N 1529, C12N 1533

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056591241

ABSTRACT:
The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed.
The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

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