Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-01-03
1997-08-19
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
435 691, 435 701, 4351723, 536 236, 536 2372, 536 241, 536 245, 800250, 47 58, 47DIG1, A01H 500, C12N 1582, C12N 1529, C12N 1533
Patent
active
056591241
ABSTRACT:
The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed.
The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.
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Crossland Lyle D.
Stein Jeffrey I.
Tuttle Annmarie
Novartis Corporation
Pace Gary M.
Robinson Douglas W.
Wai Thanda
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