Transgenic maize encoding an endosperm specific prolamin box...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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Reexamination Certificate

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10190438

ABSTRACT:
The invention provides a fertile transgenic monocot, e.g., maize, plant encoding an endosperm specific prolamin box binding factor peptide (PBF) that is expressed so as to increase the methionine and/or lysine content of the seeds of said plant over said content in the seeds of the corresponding plant that lacks said gene, wherein said corresponding plant was used to prepare said transgenic plant, or an ancestor of said transgenic plant.

REFERENCES:
patent: 5508468 (1996-04-01), Lundquist et al.
patent: 5990384 (1999-11-01), Bagga et al.
Yanagisawa et al., Involvement of Maize Dof Zinc Finger Proteins In Tissue-Specific and Light-Regulated gene Expression, The Plant Cell, 1998, 10:75-89.
Mena, Montana.,et al. ,“An endosperm-specific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin-box of a native B-hordein promoter in barley endosperm”,Plant Journal, vol. 16, No. 1, XP002165470, (Oct. 1998),53-62.
Vicente-Carbajosa, Jesus., et al. ,“A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2”,Proceedings of the Nat'l Aca of Sciences of the US, vol. 94, No. 14, XP002165471, (1997),7685-7690.
Wang, Zhendong.,et al. ,“Characterization of the maize prolamin box-binding factor-1 (PBF-1) and its role in the developmental regulation of the zein multigene family”,Gene, NL, Elsevier Biomedical Press, Amsterdam, vol. 223, No. 1-2, XP004153604, (Nov. 26, 1998),321-332.
Wang, Zhendong.,et al. ,“Modulation of gene expression by DNA-Protein and protein—protein interactions in the promoter region of the zein multigene family”,Gene, NL, Elsevier Biomedical Press, Amsterdam, vol. 223, No. 1-2, XP004153605, (Nov. 26, 1998),333-345.

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