Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via retrovirus
Reexamination Certificate
2006-11-21
2006-11-21
Falk, Anne-Marie (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
Via retrovirus
C800S008000, C800S014000, C800S021000
Reexamination Certificate
active
07138562
ABSTRACT:
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
REFERENCES:
patent: WO 90/08832 (1990-08-01), None
Haskell et.al.; Efficient Production of Transgenic Cattle by Retroviral Infection of Early Embryos, 1995, Molecular Reproduction and Deveiopment 40: 386-390.
Yee et.al.; Generation of High-Titer Pseudottped Retroviral Vectors with Very Broad Host Range, 1994, Methods In Cell Biology, vol. 43: 99-112.
Friedrich, G., et al., “Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice,” Genes and Development 5:1513-1523 (1991).
Lock, L.F., et al., “Studies of the mechanism of spontaneous germline ecotropic provirus acquisition in mice,” The EMBO Journal 7:4169-4177 (1988).
Bremel Robert D.
Burns Jane C.
Chan Anthony W. S.
Falk Anne-Marie
Ton Thai-an N.
Wisconsin Alumni research Foundation
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