Transgenic amorpha-4,11-diene synthesis

Details Transgenic amorpha-4,11-diene synthesis Transgenic amorpha-4,11-diene synthesis

2006-08-15

2006-08-15

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07091027

ABSTRACT:
The present invention relates to an isolated DNA sequence encoding a polypeptide having the biological activity of amorpha-4,11-diene synthase. This DNA sequence can be used for the transformation of bacteria, yeasts and plants for the production of amorpha-4,11-diene, a specific precursor in the synthesis of artemisinin, in the respective organisms. The invention also relates to these organisms.

REFERENCES:
Attwood et al. [Comput. Chem. 2001, col. 54(4), pp. 329-339].
Ponting [Brief. Bioinform. Mar. 2001, vol. 2(1), pp. 19-29].
Sequence alignment between Accession No. AF327526 [Liu et al, 2001] and Applicants' SEQ ID No. 13.
Brodelius, P. et al., “Metabolic Engineering of Plant Secondary Metabolism: a Tool to Improve the Productivity of Plant Cell Cultures?”Abstract Papers of the American Chemical Society, p. AGFD026, abstract, Apr. 1997.
Woerdenbag, H.J. et al., “Progress in the Research ofArtemisinin-Related Antimalarials: an Update”,Pharmacy World and Science, vol. 16, No. 4, pp. 169-180, Aug. 5, 1994.
Van Geldre, E. et al., “State of the art of the Production of the Antimalarial Compound Artemisinin in Plants”,Plant Molecular Biology, vol. 33, No. 2, pp. 199-209, 1997.
Wallaart, T.E. et al., “Bioconversion of Dihydroarteannuinic Acid into the New Antimalarial Drug Artemisinin”,Pharmacy World and Science, vol. 16, No. 3, p. C4, abstract, 1994.
Vergauwe, A. et al., “Agrobacterium iumefaciens-mediated Transformation ofArtemisia annuaL. and Regeneration of Transgenic Plants”,Plant Cell Reports, vol. 15, No. 12, pp. 929-933, 1996.
Matsushita, Y. et al., “Cloning and Analysis of a cDNA Encoding Farmesyl Diphosphate Synthase fromArtemisia annua”, Gene, vol. 172, No. 2, pp. 207-209, Jun. 26, 1996.
Brodelius, P., “Metabolic Engineering of Secondary Metabolism in Vanilla Planifolia andArtemisia annua”, Books of Abstracts 211thACS National Meeting, abstract, Mar. 24-28, 1996.
Park, C. et al., “Expression, Secretion, and Processing of Rice alpha-Amylase in the YeastYarrowia lipolytica”, Journal of Biological Chemistry, vol. 272, No. 11, pp. 6876-6881, 1997.
Chang, C. et al., “Improvement of Heterologous Protein Prouctivity Using RecombinantYarrowia lipolyticaand Cyclic Fed-Batch Process Strategy”,Biotechnology and Bioengineering, vol. 59, No. 3, pp. 379-385, 1998.
Shimada, H. et al., “Increased Carotenoid Production by the Food YeastCandida utilisthrough Metabolic Engineering of the Isoprenoid Pathway,”Applied and Environmental Microbiology, vol. 64, No. 7, pp. 2676-2680, Jul. 1998.
Müller, S. et al., “Comparison of Expression Systems in the YeastsSaccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Clonging of Two Novel Promoters fromYarrowia Lipolytica,” Yeast, 14, pp. 1267-1283, 1998.
Park, C.S. et al., “Expression, Secretion, and Processing of Rice α-Amylase in the YeastYarrowia lipolytica,” The Journal of Biological Chemistry, vol. 272, No. 11, pp. 6876-6881, Mar. 1997.
Tharaud, C. et al., “Secretion of human blood coagulation factor XIIIa by the yeastYarrowia lipolytica,” Gene, 121, pp. 111-119, 1992.
Chen, D.-C. et al., “One-step transformation of the dimorphic yeastYarrowia lipolytica,” Appl. Microbiol Biotechnol, 48, pp. 232-235, 1997.
Bohlmann, F. et al., “Cadina-4,11-Diene fromViguiera Oblongifolia,” Phytochemistry, vol. 23, No. 5, pp. 1183-1184, 1984.
Matsushita, Y. et al., “Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase fromArtemisia annua,” Gene, 172, pp. 207-209, 1996.
Back, K. et al., “Expression of a Plant Sesquiterpene Cyclase Gene inEscherichia coli,” Archives of Biochemistry and Biophysics, vol. 315, No. 2, pp. 527-532, Dec. 1994.
Facchini, P. et al., “Gene family for an elicitor-induced sesquiterpene cyclase in tobacco,”Proc. Natl. Acad. Sci. USA, vol. 89, pp. 11088-11092, Nov. 1992.
Back, K. et al., “Cloning and Bacterial Expression of a Sesquiterpene Cyclase fromHyoscyamus muticusand Its Molecular Comparison to Related Terpene Cyclases,”The Journal of Biological Chemistry, vol. 270, No. 13, pp. 7375-7381, Mar. 1995.
Omirulleh, S. et al, “Activity of a chimeric promoter with the doubled CaMV 35S enhancer element in protoplast-derived cells And transgenic plants in maize,”Plant Molecular Biology, 21, pp. 415-428, 1993.
Vergauwe, A. et al., “Agrobacterium tumefaciens-mediated transformation ofArtemisia annuaL. and regeneration of transgenic plants,”Plant Cell Reports, 15, pp. 929-933, 1996.
Hohn, T. et al., “Expression of a Fungal Sesquiterpene Cyclase Gene in Transgenic Tobacco,”Plant Physiol., 97, pp. 460-462, 1991.
Yanisch-Perron, C. et al., “Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 Vectors,”Gene, pp. 103-119, 1985.
Rogers, S.G. et al., “Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors,”Methods in Enzymology, vol. 118, pp. 627-640, 1986.
Koepp, A.E. et al., “Cyclization of Geranylgeranyl Diphosphate to Taxa-4(5), 11(12)-diene Is the Committed Step of Taxol Biosynthesis in Pacific Yew,”The Journal of Biological Chemistry, vol. 270, No. 15, pp. 8686-8690, Apr. 1995.

Affiliated with

Also associated with

Rating

Transgenic amorpha-4,11-diene synthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transgenic amorpha-4,11-diene synthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic amorpha-4,11-diene synthesis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3684190