Transgene expression system for increased persistence

Details Transgene expression system for increased persistence Transgene expression system for increased persistence

1997-04-14

1999-11-09

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Vector, per se

C12N 1500

Patent

active

059812753

ABSTRACT:
The invention is directed to a transgene expression system comprising a transcription unit which contains a transgene operably linked to expression control sequences, preferably the CMV promoter, and which is delivered simultaneously with all or part of the adenovirus E4 genomic region to a cell in order to facilitate persistent expression of the transgene. The components of the transgene expression system can be delivered by vectors including plasmids and/or viruses and may be complexed with cationic amphiphiles to facilitate entry into a cell. The invention is also directed to methods for the production of the transgene expression system. The invention is further directed to compositions that contain the transgene expression system and to methods for the use of such compositions to deliver transgenes encoding biologically active proteins to cells.

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