Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1988-08-16
1992-08-11
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4353201, 435256, 4351723, 435942, 935 28, 935 37, 935 69, C12N 910, C12N 1563, C12N 118, C12N 1581
Patent
active
051378221
ABSTRACT:
Transformed yeasts with yeast expression plasmid containing yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, which produce bovine adrenocortical cytochrome P-450.sub.17.alpha., and processes for producing 17-hydroxypregnenolone and 17-hydroxyprogesterone which comprises 17-hydroxylating pregnenolone or progesterone to 17-hydroxypregnenolone or 17-hydroxyprogesterone, respectively, with the said transformed yeasts.
REFERENCES:
patent: 4766068 (1988-08-01), Oeda
K. Yanagibashi et al. (1986) J. Biol. Chem. 261:8429-8433.
Sakaki et al., "Expression of Bovine Cytochrome P450c21 and Its Fused Enzymes with Yeast NADPH-Cytochrome P450 Reductase in Saccharomyces cerevisiae", DNA and Cell Biology, 1990, vol. 9 No. 8, pp. 603-614.
Sakaki et al., "Expression of Bovine Cytochrome P450c17 cDNA in Sacharomyces cerevisiae", DNA, 1989, vol. 8, No. 6, pp. 409-418.
Ammerer, Methods in Enzymol. 101, 192 (1983), "Expressions of Genes in Yeast Using the ADCl Promoter".
Zuber et al., J. Biol. Chem., 261, 2475 (1986), "Bovine Adrenocortical Cytochrome P-450.sub.17.alpha. ".
Zuber et al., Science, 234, 1258 (1986) "Expression of Bovine 17-Hydroxylase Cytochrome P-450 cDNA in Nonsteroidogenic (COSl) Cells".
Oeda et al., DNA, 4, 203 (1985) "Expression of Rat Liver Cytochrome P-45OMC cDNA in Saccharomyces cerevisiae".
Sakai et al., J. Biochem., 99, 741 (1986) "Monooxygenase Activity of Saccharomyces cerevisiae Cells Transformed with Expression Plasmids Carrying Rat Cytochrome P-450MC cDNA".
Ohkawa Hideo
Sakaki Toshiyuki
Shibata Megumi
Yabusaki Yoshiyasu
Director-General of Agency of Industrial Science & Technology an
Railey II Johnny F.
Schwartz Richard A.
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