Transformed Shigella

Details Transformed Shigella Transformed Shigella

2006-07-24

2008-10-21

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S252300, C424S093100

Reexamination Certificate

active

07439053

ABSTRACT:
A method for modifying a wild strain of an entero-invasiveShigellato produce a modified strain ofShigellathat can be used for making a vaccine against the wild strain ofShigella. The genome of the wild strain ofShigellais transformed so that it cannot substantially invade cells of a human host and cannot spread substantially within infected cells and from infected to uninfected cells of the host and cannot produce toxins which will kill substantial numbers of the host's infected, as well as uninfected, cells. A first gene of the wild strain ofShigella, coding for a protein necessary for theShigellato invade cells of the host, and a second gene, coding for a protein necessary for theShigellato spread within infected cells and between the infected and uninfected cells of the host, are mutagenized.

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