Transformed microorganism and process for producing...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S252330, C435S320100, C435S069100, C435S252300, C536S023100, C536S023200

Reexamination Certificate

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06943004

ABSTRACT:
A transformed microorganism prepared by inserting into a host microorganism with zinc tolerance a D-aminoacylase-producing gene which selectively produces D-aminoacylase alone between D-aminoacylase and L-aminoacylase. A process comprising culturing the transformed microorganism in a culture medium containing zinc ion and obtaining D-aminoacylase from the culture at a high efficiency.

REFERENCES:
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patent: 6030823 (2000-02-01), Tokujama et al.
patent: 6514742 (2003-02-01), Mitsuhashi et al.
patent: 62-163689 (1987-07-01), None
patent: 90/02177 (1990-03-01), None
M. Wakayama et al.: “Purification and characterization of L-aminoacylase fromPseudomonas maltophilaB1” Journal of Fermentation and Boengineering, vol. 85, No. 3, pp. 278-282 1998.
M. Wakayama et al.: “Cloning and sequencing of a gene encoding D-aminoacylase fromAlcaligenes xylosoxydanssubsp.xylosoxydansA-6 and expression of the gene inEscherichia coli” Bioscience, Biotechnology and Biochemistry, vol. 59, No. 11, pp. 2115-2119 1995.
M. Wakayama et al.: “Overproduction of D-aminoacylase fromAlcaligenes xylosoxydanssubsp.xylosoxydansA-6 inEscherichia coliand its purification” Protein Expression and Purification, vol. 7, No. 4, pp. 395-399 1996.
J-M. Farah et al.: “Mechanistic analysis of the argE-encoded N-acetylomithine deacetylase” Biochemistry, vol. 39, No. 6, pp. 1285-1293 Dec. 2000.
M. Wakayama et al.:“Role of conserved histidine residues in D-aminoacylase fromAlcaligenes xylosoxydanssubsp.xylosoxydansA-6” Bioscience, Biotechnology, and Biochemistry, vol. 64, No. 1, pp. 1-8 Jan. 2000.

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