Transformed fungal strains free of selection marker

Details Transformed fungal strains free of selection marker Transformed fungal strains free of selection marker

1998-11-10

2000-05-16

Brusca, John S.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435440, 4353201, 536 2374, C12N 115, C12N 1563, C12N 1531

Patent

active

060636149

ABSTRACT:
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.

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