Transformed cell with enhanced sensitivity to antifungal...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252300, C435S194000, C536S023200

Reexamination Certificate

active

07888058

ABSTRACT:
The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like.

REFERENCES:
patent: 5939306 (1999-08-01), Alex et al.
patent: WO 98/44148 (1998-10-01), None
Alex, Lisa et al., “Hyphal development inNeurospora crassa: Involvement of a two-component histidine kinase” Proc. Natl. Acd. Sci. USA (1996) 93:3416-3421.
Schumacher, Marc, etal., The Osmotic-1 Locus ofNeurospora crassaEncodes a Putative Histidine Kinase Similar to Osmosensors of Bacteria and Yeast, Current Microbiology (1997) 34:340-347.
Oshima, Michiyo et al., “A Point Mutation in the Two-Component Histidine KinaseBc0S-1Gene Confers Dicarboximide Resistance in Field Isolates ofBotrytis cinerea” Phytopathology (2002) 92:75-80.
Fujimura, Makoto et al, “Sensitivity to Phenylpyrrole Fungicides and Abnormal Glycerol Accumulation inOsandCutMutant Strains ofNeuropora crassa” J. Pesticide Sci (2000) 25:31-36.
Fujimura, Makoto et al., “Fungicide Resistance and Osmotic Stress Sensitivity inosMutants ofNeurospora crassa”Pesticide Biochem. Physiol. (2000) 67:125-133.
Maeda, Tatsuya et al, A two-component system that regulates an osmosensing MAP kinase cascade in yeast, Nature (1994) 369:242-245.
Aoyama, Keisuke et al., “Genetic Analysis of the His-to-Asp Phosphorelay Implicated in Mitotic Cell Cycle Control: Involvement of Histidine-Kinase Genes ofSchizosaccharomyces pombe” Biosci. Biotechnol. Biochem. (2001) 65:2347-2352.
Yamada, Hisami et al., “The Arabidopsis AHK4 Histidine Kinase is a Cytokinin-Binding Receptor that Transduces Cytokinin Signals Across the Membrane,” Plant Cell Physiol. (2001) 42:107-113.
Freeman, Jeremy et al., A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing inVibrio harveyiMol. Microbiol. (2000) 35:139-149.
Inoue, Tsutomu et al., Identification of CRE1 as a cytokinin receptor fromArabidopsisNature (2001) 409:1060-1063.
Srikantha, Thyagarajan et al, “The two-component hybrid kinase regulatorCaNIK1ofCandida albicans, ”Microbiology (1998) 144:2715-2729.
Nagahashi, Shigehisa et al, “Isolation ofCaSLNIandCaNIK1, the genes for osmosensing histidine kinase homologues, from the pathogenic fungusCandida albicans, ”Microbiology (1998) 144:425-432.
Ochiai, Noriyuki et al., Characterization of mutation in the two-component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in theos-1mutants ofNeurospora crassa, Pest Management Sci., (2001) 57:437-442.
Miller, Tamara et al, Molecular Dissection of Alleles of theosmotic-1Locus ofNeurospora crassa, Fungi Gen. Biol. (2002) 35:147-155.
GenBank Accession U50263.
GenBank Accession U53189.
GenBank Accession AAB03698.
GenBank Accession AAB01979.
GenBank Accession AF396827.
GenBank Accession AF435964.
GenBank Accession AAL37947.
GenBank Accession AAL30826.
GenBank Accession AB041647.
GenBank Accession BAB40947.
Cui, Wei et al, An osmosensing histidine kinase mediates dicarboximide fungicide resistance inBotryotinia fuckeliana(Botrytic cinerea), Fung. Gen. Biol (2002) 36:187-198.
Zhang, Yan et al, Osmoregulation and Fungicide Resistance: theNeurospora crassa os-2Gene Encodes aHOGIMitogen-Activated Protein Kinase Homologue, Appl. Environ. Microbial (2002 68:532-538 in.
Urao, Takeshi et al, “A Transmembrane Hybrid-Type Histidine Kinase in Arabidopsis Functions as an Osmosensor,” The Plant Cell, (1999) 11:1743:1754.
Gen Ban Accession U61838.
GenBank Accession U59310.
Song, Hyun Kyu, Insights into Eukaryotic Multistep Phosphorelay Signal Transduction Revealed by the Crystal Structure of Ypd1p fromSaccharomyces cerevisiae, J. Mol. Biol. (1999) 293, 753-761.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Transformed cell with enhanced sensitivity to antifungal... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transformed cell with enhanced sensitivity to antifungal..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transformed cell with enhanced sensitivity to antifungal... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2631583

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.