Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1987-06-15
1991-06-18
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
43524049, 4352405, 43524054, 935 67, C12N 1500, C12N 500
Patent
active
050249444
ABSTRACT:
A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed.
Methods for transforming somatic tissue from soybean and other Glycine species are also provided.
Whole, fertile, transformed plants are obtained.
REFERENCES:
patent: 4684612 (1987-08-01), Hemphill
Barwale et al., 1986, Planta 167:473-481.
Kameya et al., 1981, Plant Sci. Lett. 21:289-294.
Sengupta-Gopalan et al., 1985, Proc. Natl. Acad. Sci., U.S.A., 82:3320-3324.
De Block et al., 1984, EMBO J, 3(8):1681-1689.
Owens et al., 1985, Plant Physiol., 77:87-94.
Lippmann et al., 1984, Plant Cell Rep., 3:215-218.
Ranch et al., 1985, In Vitro, 21(11):653-658.
Raquin, C., 1983, Z. Pflanzenphysiol., 111:453-457.
Lazzeri et al., 1985, Plant Mol. Biol. Rep., 3(4):160-167.
Barwale, U. B. (1985), "Screening of soybean cultivars for plant regeneration of soybean plants from undifferentiated tissue," Master's Thesis, cataloged by the University of Illinois Library, Mar. 16, 1986, p. 59.
Hood, E. E. et al. (1984), Bio/Technology, 2:702-709.
Hood (1986), J. Bact., 168:1283-1290.
Wyndaele, R. et al. (1985), Plant Cell Physiol., 26:1147-1154.
Bialy, H. (1985), Bio/Technology, 3:200-201.
Kudirka, D. T. et al. (1986), Can. J. Genet. Cytol., 28:808-817.
Simpson, R. B. et al. (1986), Plant Mol. Biol., 6:403-415.
Byrne, M. C. et al. (1987), Plant Cell. Tissue and Organ Culture, 8:3-15.
Shahin, E. A. and Simpson, R. B. (1986), Abstract for presentation at the Conference on Molecular and Cellular Biology of the Soybean, Ames, Iowa.
Williams, E. G. and Maheswaran, G. (1986), Annals of Bot., 57:443-462.
Adams Thomas R.
Collins Glenn B.
Hartweck Lynn M.
Hildebrand David F.
Lazzeri Paul A.
Fox David T.
Lubrizol Genetics Inc.
The University of Kentucky Research Foundation, Inc.
Weimar Elizabeth C.
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