Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion
Reexamination Certificate
2003-07-07
2010-02-16
Worley, Cathy Kingdon (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Involving particle-mediated transfecion
C800S288000, C800S292000, C800S296000, C435S468000
Reexamination Certificate
active
07663026
ABSTRACT:
This invention relates to marine algae, and more particularly, to a method for producing improved seaweed strains by genetic engineering. The vector for transformation were constructed by inserting the high-plant or algae-derived promoters upstream of foreign reporter genes or such cassettes that functional genes are fused with antibiotics or herbicide-resistant genes. The genetic seaweed was generated by natural development process by recombinated plasmid DNA introduction to seaweed spore with Biolostics as transformation methods. Introduced traits of antibiotics or herbicide-resistance were used to select the transgenic seaweed individuals when foreign functional genes are transformed. Stable transformation could be obtained following this invention.
REFERENCES:
patent: 1157326 (1992-08-01), None
Song et al. A transformation model forLaminaria japonica(Phaeophyta, Laminariales). (1998) Chin. J. Oceanol. Limnol.; vol. 16, Suppl.; pp. 50-55.
Jiang, et al., “Expression of Hepatitis B Surface Antigen Gene (HBsAg) inLaminaria japonica(Laminariales, Phaeophyta),” Chin. Sci. Bullet. 2002, 47(17): 1095-1097. (English Abstract Only).
Yu et al., “Transient Expression of lacZ Reporter Gene In the Economic SeaweedUndaria pinnatifida,” High Technol. Letters 2002, 12(8):93-95. (English Abstract Only).
Jiang Peng
Li Fuchao
Qin Song
Sun Guoqiong
Yu Daozhan
Institute of Oceanology Chinese Academy of Sciences
Kingdon Worley Cathy
Smith , Gambrell & Russell, LLP
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