Transformation of plants to introduce closely linked markers

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 435 701, 47 58, C12N 1500, C12P 2100, C12P 2104, A01H 100

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active

052780570

ABSTRACT:
A novel method of producing a plant with a marker closely linked to a target locus, in particular a nuclear male sterile target locus, is described. The method involves transformation of a group of plants in order to introduce a marker into each plant, and isolation of a plant with the marker closely linked to a target locus. The markers include visible markers and dominant conditional lethal markers. The method is of particular use for hybrid seed production where the target locus is a nuclear male sterile locus.

REFERENCES:
Goodman et al. 1987, Science 236:48-54.
DeBlock et al. 1984, EMBO J 3(8):1681-1689.
Horsch et al. 1984, Science 223:496-498.

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