Transformation method for plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

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800295, 800298, 435419, 435468, 4353201, 536 236, 536 237, 536 241, C12N 1500, C12N 1529, C12N 1582, A01H 400

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061405531

ABSTRACT:
A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of:

REFERENCES:
Bolton et al. Science. vol. 232, pp. 983-985, May 1986.
Stachel et al. Nature. vol. 318, No. 19, pp. 624-629, Dec. 26, 1985.
Jacq et al. Plant Cell Reports. vol. 12, pp. 621-624, 1993.
S. Sheikholeslam et al., "Acetosyringone Promotes High Efficiency Transformation of Arabidopsis thaliana Explants by Agrobacterium tumefaciens", Plant Molecular Biology 8:291-298 (1987).
W. Schafer et al., "T-DNA Integration and Expression in a Monocot Crop Plant After Induction of Agrobacterium", Nature, 327:529-533 (1987).
D. Raineri et al., "Agrobacterium-Mediated Transformation of Rice (Oryza Sativa L.)", Biotechnology 8:33-38 (1990).
Wen-Hui Shen et al., "T-DNA Transfer to Maize Cells: Histochemical Investigation of .beta.-Glucuronidase Activity in Maize Tissues", Proc. Natl. Acad. Sci. USA, 90:148-1492 (1993).

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