Transformation and regeneration of sunflower cotyledons

Details Transformation and regeneration of sunflower cotyledons Transformation and regeneration of sunflower cotyledons

2006-02-14

2006-02-14

McElmain, Elizabeth (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S294000, C800S322000

Reexamination Certificate

active

06998516

ABSTRACT:
The present invention relates to improved methods for producing transgenic sunflower plants. Specifically, methods are disclosed for transforming sunflower tissues and regenerating fertile transgenic plants therefrom. The methods are particularly amenable to sunflower tissues, such as cotyledons, which have proven refractory to previous methods of transformation and regeneration.

REFERENCES:
patent: 6130368 (2000-10-01), Londesborough et al.
Hansen et. al., 1999, Trends in plant Science, vol. 4, pp. 226-231, see p. 230.
Ceriani, M.F. et al, “Cotyledons: an explant for routine regeneration of sunflower plants,” Plant Cell Physiol., vol. 33 (2), p. 157-164, (1992).
Coca, M.A. et al, “Differential regulation of small heat shock genes in plants: analysis of a water stress inducible and developmentally activated sunflower promoter,” Plant Mol. Biol., vol. 31, p. 863-876, (1996).
Goffner, D. et al, “Effects of abscisic acid and osmotica on Helianthinin gene expression in sunflower cotyledons in vitro,” Plant Sci., vol. 66, p. 211-219, (1990).
Grayburn, W.S. et al, “Transformation of sunflower (Helianthus annuusL.) following wounding with glass beads,” Plant Cell Reports, vol. 14, p. 285-289, (1995).
Hammond, R.W, “Agrobacterium mediated inoculation of PSTVd cDNAs onto tomato reveals the biological effect of apparently lethal mutations,” Virology, vol. 201, p. 36-45, (1994).
Kutschera, U. et al, “Cell elongation, turgor and osmotic pressure in developing sunflower hypocotyls,” J. Exp. Botany, vol. 45 (No. 274), p. 591-595, (1994).
Kutschera, U, “Cell expansion in plant development,” Revista Brasileira de Fisiologia Vegetal, vol. 12 (1), p. 65-95, (Apr. 2000).
Kutschera, U, “Role of the cotyledons in the maintenance of hypocotyl growth inHelianthus annuusL., ” Plant Physiol., vol. 140, p. 319-323, (1992).
Laparra, H. et al, “Expression of foreign genes in sunflower (Helianthus annuusL.): evaluation of three gene transfer methods,” Euphytica, vol. 85, p. 63-74, (1995).
McNeil, D.L., “The basis of osmotic pressure maintenance during expansion growth inHelianthus annuushypocotyls,” Aust. J. Plant Physiol., vol. 3 (3), p. 311-324, (1976).
Pfeiffer, I. et al, “Sucrose metabolism and lipid mobilization during light induced expansion of sunflower cotyledons,” J. Plant Physiol., vol. 147, p. 553-558, (1996).
Rao, K.S. et al, “Agrobacterium mediated transformation of sunflower (Helianthus annuusL.): a simple protocol,” Annals of Botany, vol. 83, p. 347-354, (1999).
Xu, Y. et al, “Transfer and expression of the T-DNA haboured by Agrobacterium tumefaciens in cultured explantsHelianthus annuus,” Acta Bio. Yunnanica, vol. 10(1), p. 159-166, (1988).

Affiliated with

Also associated with

Rating

Transformation and regeneration of sunflower cotyledons does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Transformation and regeneration of sunflower cotyledons, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transformation and regeneration of sunflower cotyledons will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3641612